CD40/TNFRSF5 Antibody (5C3) - BSA Free

Novus Biologicals | Catalog # NBP1-43416

Clone 5C3 was used by HLDA to establish CD designation.
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5C3

Format

BSA Free
View all CD40/TNFRSF5 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD40.

Reactivity Notes

Mouse reactivity reported in scientific literature (Chechneva O et al).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD40/TNFRSF5 Antibody (5C3) - BSA Free

Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]

Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]

Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the PE conjugate of NBP1-43416. Analysis of human peripheral blood leukocytes using CD40 PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.
Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]

Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]

Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the FITC conjugate of NBP1-43416. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (blue histogram) or Anti-Human CD40 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

Applications for CD40/TNFRSF5 Antibody (5C3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500
Application Notes
The 5C3 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. This antibody may also be used for immunohistochemical staining of frozen tissue sections. 5C3 has also been reported in in vitro functional studies.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD40/TNFRSF5

The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.

Alternate Names

CD40, TNFRSF5

Gene Symbol

CD40

Additional CD40/TNFRSF5 Products

Product Documents for CD40/TNFRSF5 Antibody (5C3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD40/TNFRSF5 Antibody (5C3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD40/TNFRSF5 Antibody (5C3) - BSA Free

Customer Reviews for CD40/TNFRSF5 Antibody (5C3) - BSA Free

There are currently no reviews for this product. Be the first to review CD40/TNFRSF5 Antibody (5C3) - BSA Free and earn rewards!

Have you used CD40/TNFRSF5 Antibody (5C3) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD40/TNFRSF5 Antibody (5C3) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I wanna know does CD40 antibody for IHC-P comes with positive control slide?

    A: Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.

  • Q: We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?

    A: Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

  • Q: I wanna know does CD40 antibody for IHC-P comes with positive control slide?

    A: Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.

  • Q: We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?

    A: Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies