CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin - Low Endotoxin, Azide and BSA Free

Novus Biologicals | Catalog # NBP2-62227

Clone MEM-59 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-59

Format

Low Endotoxin, Azide and BSA Free
View all CD43 Primary Antibodies »

Product Specifications

Immunogen

Human T lymphocytes.

Specificity

The antibody MEM-59 recognizes a neuraminidase-sensitive extracellular epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein

Scientific Data Images

Western Blot: CD43/Sialophorin Antibody (MEM-59)Low Endotoxin [NBP2-62227]

Western Blot: CD43/Sialophorin Antibody (MEM-59)Low Endotoxin [NBP2-62227]

Western Blot: CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-human CD43 (MEM-59).Lane 1: lysate of human PBLLane 2: lysate of canine PBLLane 3: lysate of porcine PBL
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227]

Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227]

Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Staining of human spleen (paraffin sections) using anti-CD43 (MEM-59).
Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227]

Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227]

Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) PE.
Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227]

Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227]

Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) APC-CyTM7.
Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227]

Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227]

Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) biotin / streptavidin-APC.
Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227]

Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227]

Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) FITC.
CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227]

Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227]

Staining of human spleen (paraffin sections) using anti-CD43 (MEM-59).Commercially tested by LifeSpan BioSciences.

Applications

Application
Recommended Usage

Flow Cytometry

1-5 ug/ml

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Functional application: The antibody MEM-59 activates T lymphocytes and also induces apoptosis in hemopoietic progenitor cells. Immunohistochemistry (paraffin sections): Positive tissue: spleen, thymus, tonsil.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

Low Endotoxin, Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD43

CD43 is a key glycoprotein of thymocytes and T lymphocytes, which affects the physicochemical properties of the T-cell surface and plays a role in lectin binding. This antibody contains an extended rodlike structure that may protrude above the glycocalyx of the cell, allowing accessibility of multiple glycan chains for binding. During T-cell activation CD43 is actively removed from the T-cell-antigen-presenting cell contact site, thus suggesting CD43's role in negative regulation of the adaptive immune response.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Gene Symbol

SPN

Additional CD43 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin - Low Endotoxin, Azide and BSA Free

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Protocols

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FAQs

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