Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # W3/13

Format

BSA Free
View all CD43 Primary Antibodies »

Product Specifications

Immunogen

Rat thymocyte membrane glycoproteins

Reactivity Notes

Reacts with Rat.

Localization

Type I membrane protein.

Specificity

Rat CD43 cell surface antigen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD43/Sialophorin Antibody (W3/13) - BSA Free

Western Blot: CD43/Sialophorin Antibody (W3/13)BSA Free [NB100-64992]

Western Blot: CD43/Sialophorin Antibody (W3/13)BSA Free [NB100-64992]

Western Blot: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Western blot shows lysates of rat lymph node, rat spleen tissue, and rat thymus tissue. PVDF membrane was probed with 0.5 ug/mL of Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # NB100-64992) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD43 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions.
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (W3/13) - BSA Free [NB100-64992]

Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (W3/13) - BSA Free [NB100-64992]

Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - CD43 was detected in immersion fixed paraffin-embedded sections of rat thymus using Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # NB100-64992) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was incubated with VisUBlock™ Mouse on Mouse Blocking Reagent (Catalog # VB001) and then subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.
Flow Cytometry: CD43/Sialophorin Antibody (W3/13) - BSA Free [NB100-64992]

Flow Cytometry: CD43/Sialophorin Antibody (W3/13) - BSA Free [NB100-64992]

Flow Cytometry: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Staining of rat splenocytes using CD43 FITC.

Applications for CD43/Sialophorin Antibody (W3/13) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5 ug/ml
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Use in Western blot reported in scientific literature (PMID 7504163).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD43

CD43 is one of the major glycoproteins of thymocytes and T lymphocytes, it plays a role in the physicochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. The antigen is a counter receptor for SN/Siglec1. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site suggesting a negative regulatory role in adaptive immune response. CD43 is expressed by the majority of leucocytes, with the exception of most B cells. However CD43 expression is seen in some cases of B cell lymphocytic lymphoma and centrocytic lymphoma. CD43 cell surface antigen, also known as leukosialin and sialophorin. CD43 is a 95kD heavily glycosylated glycoprotein expressed by all leucocytes with the exception of B lymphocytes. This product is routinely tested in flow cytometry on rat splenocytes.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Gene Symbol

SPN

Additional CD43 Products

Product Documents for CD43/Sialophorin Antibody (W3/13) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD43/Sialophorin Antibody (W3/13) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD43/Sialophorin Antibody (W3/13) - BSA Free

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Protocols

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