Key Product Details

Species Reactivity

Mouse

Applications

ELISA Detection (Matched Antibody Pair), Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 341 expressed in HEK293

Format

Azide and BSA Free
View all CD48/SLAMF2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD48/SLAMF2 (Accession#: NP_031675.1; Met1-Arg216).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD48/SLAMF2 Antibody (341) - Azide and BSA Free

Flow Cytometry: CD48/SLAMF2 Antibody (341) [NBP2-90577]

Flow Cytometry: CD48/SLAMF2 Antibody (341) [NBP2-90577]

Flow Cytometry: CD48/SLAMF2 Antibody (341) [NBP2-90577] - Flow cytometric analysis of Mouse CD48/SLAMF2 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD48/SLAMF2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
CD48/SLAMF2 Antibody (341) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CD48/SLAMF2 Antibody (341) [NBP2-90577] -

Immunocytochemistry/Immunofluorescence: CD48/SLAMF2 Antibody (341) [NBP2-90577] - Immunofluorescence staining of Mouse CD48/SLAMF2 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90577 antibody (1:60) at 37C 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cell membrane.

Applications for CD48/SLAMF2 Antibody (341) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100
Application Notes
This antibody will detect Human CD48/SLAMF2 in ELISA pair set (Catalog: # NBP2-79582).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD48/SLAMF2

CD48 is a glycosyl phophatidyl inositol (GPI) anchored cell surface protein also known as Blast1, HuLy m3, BCM1 (in mouse), and OX45 (in rat). CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibrolasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. The CD48 antigen is closely related to the activation antigen, Blast 1.

Long Name

SLAM Family Member 2

Alternate Names

BCM1, BLAST1, CD48, MEM-102, SLAMF2

Gene Symbol

CD48

Additional CD48/SLAMF2 Products

Product Documents for CD48/SLAMF2 Antibody (341) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD48/SLAMF2 Antibody (341) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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