CD52 Antibody (Campath-1H) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-75896
![Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]](https://resources.rndsystems.com/images/products/CD52-Antibody-Campath-1H-Immunohistochemistry-NBP2-75896-img0006.jpg "Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]")
Key Product Details
Species Reactivity
Human, Cynomolgus Monkey, Rhesus Macaque
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vivo assay
Label
Unconjugated
Antibody Source
Monoclonal Human IgG1 kappa Clone # Campath-1H
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human lymphocytes
Specificity
Human CD52 antigen, also known as CAMPATH-1. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
Clonality
Monoclonal
Host
Human
Isotype
IgG1 kappa
Scientific Data Images for CD52 Antibody (Campath-1H) - Azide and BSA Free
Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]
Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Anti-CD52 staining of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (NBP2-75896) was used to stain samples at a concentration of 5 ug/ml.![Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]](https://resources.rndsystems.com/images/products/CD52-Antibody-Campath-1H-Immunohistochemistry-NBP2-75896-img0003.jpg "Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]")
Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]
Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Formalin fixed human thyroid cancer slices were were stained with a NBP2-75896 at 5 ug/ml.![Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]](https://resources.rndsystems.com/images/products/CD52-Antibody-Campath-1H-Immunohistochemistry-NBP2-75896-img0004.jpg "Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]")
Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]
Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Anti-CD52 staining of formaldehyde fixed paraffin embedded rat epididymis tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (NBP2-75896) was used to stain samples at a concentration of 5 ug/ml.![Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]](https://resources.rndsystems.com/images/products/CD52-Antibody-Campath-1H-Immunohistochemistry-NBP2-75896-img0005.jpg "Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]")
Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]
Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Anti-CD52 staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (NBP2-75896) was used to stain samples at a concentration of 5 ug/ml.![Immunofluorescence: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]](https://resources.rndsystems.com/images/products/CD52-Antibody-Campath-1H-Immunofluorescence-NBP2-75896-img0002.jpg "Immunofluorescence: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]")
Immunofluorescence: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]
Immunofluorescence: CD52 Antibody (Campath-1H) [NBP2-75896] - Formaldehyde-fixed rat thymus slices were stained with NBP2-75896 at 5 ug/ml and detected with a FITC-conjugated secondary antibody.Applications for CD52 Antibody (Campath-1H) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
In vivo assay
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD52
Alternate Names
CAMPATH-1, CD52, CDw52, He5, HEL-S-171mP, biosimilar antibody, biosimilars
Gene Symbol
CD52
Additional CD52 Products
Product Documents for CD52 Antibody (Campath-1H) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD52 Antibody (Campath-1H) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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