CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-22542
![Western Blot: CD58/LFA-3 Antibody (TS2/9)Azide and BSA Free [NBP2-22542]](https://resources.rndsystems.com/images/products/CD58-LFA-3-Antibody-TS2-9-Western-Blot-NBP2-22542-img0005.jpg "Western Blot: CD58/LFA-3 Antibody (TS2/9)Azide and BSA Free [NBP2-22542]")
Key Product Details
Species Reactivity
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # TS2/9
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human Leukocyte Function Antigen-3
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free
Western Blot: CD58/LFA-3 Antibody (TS2/9)Azide and BSA Free [NBP2-22542]
Western Blot: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of 25 ug of Raji (Lane 1), Jurkat (Lane 2), and BAF-3 cell lysates (Lane 3) and a molecular weight protein ladder.![Immunocytochemistry/ Immunofluorescence: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]](https://resources.rndsystems.com/images/products/CD58-LFA-3-Antibody-TS2-9-Immunocytochemistry-Immunofluorescence-NBP2-22542-img0004.jpg "Immunocytochemistry/ Immunofluorescence: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]")
Immunocytochemistry/ Immunofluorescence: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]
Immunocytochemistry/Immunofluorescence: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 (green) showing staining in the cytoplasm of Raji cells (right) compared to a negative control without primary antibody (left).![Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]](https://resources.rndsystems.com/images/products/CD58-LFA-3-Antibody-TS2-9-Flow-Cytometry-NBP2-22542-img0003.jpg "Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]")
Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]
Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 in BAF-3 cells compared to an isotype control (blue).![Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]](https://resources.rndsystems.com/images/products/CD58-LFA-3-Antibody-TS2-9-Flow-Cytometry-NBP2-22542-img0001.jpg "Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]")
Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]
Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 in PBMC cells compared to an isotype control (blue).![Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]](https://resources.rndsystems.com/images/products/CD58-LFA-3-Antibody-TS2-9-Flow-Cytometry-NBP2-22542-img0002.jpg "Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]")
Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]
Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 in PBMC cells compared to an isotype control (blue).Applications for CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:100
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Western Blot
1:100 - 1:500
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
0.98 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CD58/LFA-3
Additional CD58/LFA-3 Products
Product Documents for CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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