CD59 Antibody (MEM-43/5) - BSA Free

Novus Biologicals | Catalog # NB500-400

Clone MEM-43/5 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # MEM-43/5

Format

BSA Free
View all CD59 Primary Antibodies »

Product Specifications

Immunogen

Thymocytes and T lymphocytes.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies. HLDA V; WS Code AS S012

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD59 Antibody (MEM-43/5) - BSA Free

Western Blot: CD59 Antibody (MEM-43/5)BSA Free [NB500-400]

Western Blot: CD59 Antibody (MEM-43/5)BSA Free [NB500-400]

Western Blot: CD59 Antibody (MEM-43/5) [NB500-400] - Fig. 1. Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5). Lane 1: original cell lysate Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43).
Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43/5) - BSA Free [NB500-400]

Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43/5) - BSA Free [NB500-400]

Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43/5) [NB500-400] - Staining of human skin (paraffin sections) using anti-CD59 (MEM-43/5).
Flow Cytometry: CD59 Antibody (MEM-43/5) - BSA Free [NB500-400]

Flow Cytometry: CD59 Antibody (MEM-43/5) - BSA Free [NB500-400]

Flow Cytometry: CD59 Antibody (MEM-43/5) [NB500-400] - The PIGAc.1234C>T mutation does not impair terminal hematopoietic differentiation during mesoderm induction. Representative example of FACS analysis of hematopoietic phenotypes in the EB-BLCs from PIGAwt and PIGAc.1234C>T. The zebra plot shows expression of CD59 (X-axis) and CD45 (Y-axis) after three and eight days of hematopoietic differentiation. Unstained PIGAwt cells were used as a control. Abbreviations: CFU-Macrophage (M); CFU-Granulocyte-Macrophage (GM); committed erythroid BFU-E (BFU) and CFU-E (CFU) progenitors; multipotent progenitor cells CFU-GEMM (GEMM). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license. Data from the APC-conjugated form of anti-CD59 MEM-43/5.
Flow Cytometry: CD59 Antibody (MEM-43/5) - BSA Free [NB500-400]

Flow Cytometry: CD59 Antibody (MEM-43/5) - BSA Free [NB500-400]

Flow Cytometry: CD59 Antibody (MEM-43/5) [NB500-400] - The PIGAc.1234C>T mutation increases PIGA function compared to PIGAnull hiPSCs.(A). Representative example of FACS analysis CD59 expression in the three hiPSC lines. Overlay histogram shows that CD59 expression was significantly higher in PIGAc.1234 C>T hiPSCs compared to PIGAnull hiPSCs. MFI was 445.4 in PIGAwt hiPSCs and 332.6 in PIGAc.1234C>T hiPSCs (p>0.05, NS). However, MFI in PIGAc.1234C>T hiPSCs was significantly higher than 17.9 in PIGAnull hiPSCs (*pT hiPSCs. PIGAnull hiPSCs (purple), PIGAc.1234C>T hiPSCs (orange) and PIGAwt hiPSCs (blue). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license. Data from the APC-conjugated form of clone MEM-43/5.
Flow Cytometry: CD59 Antibody (MEM-43/5) - BSA Free [NB500-400]

Flow Cytometry: CD59 Antibody (MEM-43/5) - BSA Free [NB500-400]

Flow Cytometry: CD59 Antibody (MEM-43/5) [NB500-400] - Generation of PIGAc.1234C>T mutation using the PiggyBac transposon system. Representative FACS analysis CD59 expression in TF1PIGAnull cells transfected with PB-PIGAwt, PB-PIGAc.1234C>T or PB-PIGAtr411. Transfected TF1PIGAnull cells were stained with an APC-conjugated CD59 antibody to assess PIGA gene expression. Non-transfected TF1PIGAnull cells were used as a control. MFI represents mean fluorescence intensity. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license. Data from the APC-conjugated form of MEM-43/5.

Applications for CD59 Antibody (MEM-43/5) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-4 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:50

Western Blot

1-2 ug/ml
Application Notes
Western Blot - Non-reducing conditions. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD59

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Alternate Names

CD59, HRF20, MACIF, MIC11, Protectin

Entrez Gene IDs

966 (Human); 12509 (Mouse)

Gene Symbol

CD59

Additional CD59 Products

Product Documents for CD59 Antibody (MEM-43/5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD59 Antibody (MEM-43/5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD59 Antibody (MEM-43/5) - BSA Free

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Protocols

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