CD63 Antibody (MX-49.129.5)

Novus Biologicals | Catalog # NBP2-32830

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MX-49.129.5
View all CD63 Primary Antibodies »

Product Specifications

Immunogen

Full length CD63 of human origin (Uniprot: P08962 )

Localization

Cell Surface & Cytoplasmic

Marker

Late Endosomes Marker

Specificity

This monoclonal antibody recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of monoclonal antibody LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34689)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for CD63 Antibody (MX-49.129.5)

Western Blot: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Western Blot: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Western Blot: CD63 Antibody (MX-49.129.5) [NBP2-32830] - Exosome or whole cell lysates (WCL) sample from MDA-MB-231 cells was loaded with 10 ug/lane. 10% SDS-PAGE. CD63 antibody (NBP2-32830) was used for primary antibody: 1:500, 4C, overnight. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Immunocytochemistry/ Immunofluorescence: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Immunocytochemistry/Immunofluorescence: CD63 Antibody (MX-49.129.5) [NBP2-32830] - Immunofluorescence: - Azide and BSA Free [NBP2-34689] - IF staining of HeLa cells using AF488 labeled CD63 Monoclonal Antibody (MX-49. 129.5) (green). F-actin filaments are labeled with dylight 554 phalloidin (red) Nuclei stained with DAPI (blue).
Immunohistochemistry-Paraffin: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Immunohistochemistry-Paraffin: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Immunohistochemistry-Paraffin: CD63 Antibody (MX-49.129.5) [NBP2-32830] - Formalin-fixed, paraffin-embedded Mouse spleen stained with CD63 Antibody (MX-49.129.5)
Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830] - Flow cytometric analysis of bead-bound exosomes derived from MCF-7 cells. CD63 Mouse Monoclonal antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF568 (orange); unstained exosomes (gray).
Western Blot: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Western Blot: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Western Blot: CD63 Antibody (MX-49.129.5) [NBP2-32830] - Western Blot: CD63 Antibody (MX-49.129.5) - Azide and BSA Free [NBP2-34689] - Western blot of human spleen Lysate with CD63 Monoclonal Antibody (IMX-49. 129.5)
Immunohistochemistry-Paraffin: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Immunohistochemistry-Paraffin: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Immunohistochemistry-Paraffin: CD63 Antibody (MX-49.129.5) [NBP2-32830] - Immunohistochemistry-Paraffin: CD63 Antibody (MX-49.129.5) - Azide and BSA Free [NBP2-34689] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD63 Monoclonal Antibody (MX-49. 129.5)
Immunohistochemistry-Paraffin: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Immunohistochemistry-Paraffin: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Immunohistochemistry-Paraffin: CD63 Antibody (MX-49.129.5) [NBP2-32830] - Formalin-fixed, paraffin-embedded human melanoma stained with CD63 Antibody (MX-49.129.5)
Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830] - Flow Cytometry: CD63 Antibody (MX-49.129.5) - Azide and BSA Free [NBP2-34689] - Flow Cytometry of NIH/3T3 Cells. Black: Cells alone; Green: Isotype Control; Red; PE-labeled CD63 Monoclonal Antibody (MX-49. 129.5)
Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830] - Flow Cytometric staining of CD63 on human PBMC cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled CD63 Monoclonal Antibody (MX-49.129.5).
Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830] - Flow cytometry of MCF-7 cells unstained (gray) or stained with CF488A-labeled CD63 monoclonal antibody (MX-49.129.5) (green).
Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830] - Flow cytometry of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or stained with CF488A-labeled CD63 monoclonal antibody (MX-49.129.5) (green)
Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830]

Flow Cytometry: CD63 Antibody (MX-49.129.5) [NBP2-32830] - Flow cytometric analysis of MCF-7 cells. CD63 Mouse Monoclonal antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF568 (orange); unstained cells (gray).
CD63 Antibody (MX-49.129.5)

Western Blot: CD63 Antibody (MX-49.129.5) [NBP2-32830] -

Characterization of urine and urinary exosomes isolated by BEST of PE and HC. (A) Representative nanoparticle tracking analysis for untreated urine and isolated urine from PE and HC, depicting particle diameter and the number of particles. (B) and (C) Comparisons of particle concentration and particle size between PE and HC. (D) Western blots of TSG101 and CD63 protein extracted from isolated exosomes. (E) and (F) Quantitative analysis of exosome marker TSG101 and CD63 protein. All data are presented as mean \documentclass[12pt]{minimal}\usepackage{amsmath}\usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy}\usepackage{mathrsfs}\usepackage{upgreek}\setlength{\oddsidemargin}{-69pt}\begin{document}$$\:\pm\:$$\end{document} standard error and analyzed by paired t-test and independent two-tailed t-test. BEST, biologically intact exosome separation technology; PE, patients with preeclampsia; HC, healthy controls. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39406891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CD63 Antibody (MX-49.129.5)

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

0.5-1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 2 reviews rated 5 using NBP2-32830 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD63

CD63 (cluster of differentiation 63), also known as lysosome associated membrane protein 3 (LAMP-3), is a membrane spanning glycoprotein with a molecular mass ranging from 30-60 kDa that was the first characterized member of the tetraspanin superfamily (1,2). CD63 is ubiquitously expressed and largely present on the cell surface in the endosomal system (1-3). More specifically, it is generally present in multivesicular bodies (MVBs), also called late endosomes, and lysosomes (1). The CD63 molecule is a total of 238 amino acids (aa), has four hydrophobic membrane-spanning regions and three N-glycosylation sites, and the encoded protein has a theoretical molecular weight of 25 kDa (2,3). CD63 both directly and indirectly interacts with other proteins including integrins, cell surface receptors, other tetraspanins, kinases, and adapter proteins, to name a few (1). CD63 is involved in many cell processes including cell survival and activation, cell adhesion, invasion, and migration (1). Additionally, CD63 has been shown to interact with tissue inhibitor of metalloproteinase 1 (TIMP-1), which originally thought to be an inhibitor of cancer progression has recently been shown to have cancer promoting properties as well (1,4). The CD63-TIMP-1 interaction has been shown to activate the PI3K/AKT pathway in lung adenocarcinoma cells and also promote survival and invasion of acute myeloid leukemia cells (1).CD63 is a useful flow cytometry marker for basophil granulocytes and can be used for the basophil activation test (BAT) to assess IgE-mediated allergy response (5). As CD63 was initially discovered on activated blood platelets and is a lysosomal-associated protein it makes sense it would be involved in platelet or lysosomal-related disorders (1,2). More precisely, CD63 is associated with Hermansky-Pudlak syndrome, a disease characterized by albinism and platelet storage pool deficiency (6).

References

1. Pols, M. S., & Klumperman, J. (2009). Trafficking and function of the tetraspanin CD63. Experimental cell research. https://doi.org/10.1016/j.yexcr.2008.09.020

2. Metzelaar, M. J., Wijngaard, P. L., Peters, P. J., Sixma, J. J., Nieuwenhuis, H. K., & Clevers, H. C. (1991). CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. The Journal of biological chemistry.

3. Horejsi, V., & Vlcek, C. (1991). Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63. FEBS letters. https://doi.org/10.1016/0014-5793(91)80988-f

4. Eckfeld, C., HauBler, D., Schoeps, B., Hermann, C. D., & Kruger, A. (2019). Functional disparities within the TIMP family in cancer: hints from molecular divergence. Cancer metastasis reviews. https://doi.org/10.1007/s10555-019-09812-6

5. Hoffmann, H. J., Santos, A. F., Mayorga, C., Nopp, A., Eberlein, B., Ferrer, M., Rouzaire, P., Ebo, D. G., Sabato, V., Sanz, M. L., Pecaric-Petkovic, T., Patil, S. U., Hausmann, O. V., Shreffler, W. G., Korosec, P., & Knol, E. F. (2015). The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease. Allergy. https://doi.org/10.1111/all.12698

6. Dell'Angelica, E. C., Shotelersuk, V., Aguilar, R. C., Gahl, W. A., & Bonifacino, J. S. (1999). Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. Molecular cell. https://doi.org/10.1016/s1097-2765(00)80170-7

Alternate Names

CD63, Granulophysin, Lamp-3, ME491, OMA81H, Tspan30

Gene Symbol

CD63

UniProt

P08962

Additional CD63 Products

Product Documents for CD63 Antibody (MX-49.129.5)

Certificate of Analysis

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Product Specific Notices for CD63 Antibody (MX-49.129.5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD63 Antibody (MX-49.129.5)

Customer Reviews for CD63 Antibody (MX-49.129.5) (2)

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  • CD63 Antibody (MX-49.129.5)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231
    Species: Human
    Verified Customer | Posted 10/20/2022
    Western Blot: Exosome or whole cell lysates (WCL) sample from MDA-MB-231 cells was loaded with 10 ug/lane. 10% SDS-PAGE. CD63 Antibody (NBP2-32830) was used for primary antibody: 1:500, 4℃, overnight.
    CD63 Antibody (MX-49.129.5) NBP2-32830
  • CD63 Antibody (MX-49.129.5)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T cell lysate
    Species: Human
    Verified Customer | Posted 08/03/2022
    CD63 overexpression cells
    1:500 in TBST
    CD63 Antibody (MX-49.129.5) NBP2-32830

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FAQs for CD63 Antibody (MX-49.129.5)

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  • Q: I am wondering whether the CD62 MX-49.129.5 monoclonal antibody (NBP2-32830) is working for western blot under reducing conditions?

    A: One specific condition isn't recommended, so the CD63 antibody should work in both reducing and non reducing conditions.

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