CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80656

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional, Single Cell Western, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # FA-11

Format

Azide and BSA Free
View all CD68/SR-D1 Primary Antibodies »

Product Specifications

Immunogen

This CD68/SR-D1 Antibody (FA-11) was developed against purified ConA acceptor glycoproteins from the P815 cell line.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free

Western Blot: CD68/SR-D1 Antibody (FA-11)Azide and BSA Free [NBP2-80656]

Western Blot: CD68/SR-D1 Antibody (FA-11)Azide and BSA Free [NBP2-80656]

Western Blot: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656] - Expression on J774 cells. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656]

Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656]

Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656] - Wehi-3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti CD68 (FA-11) [NBP2-33337] at a 1:100 dilution overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656]

Immunohistochemistry: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656]

Immunohistochemistry: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656] - Mouse spleen. Image from the standard format of this antibody.
Flow Cytometry: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656]

Flow Cytometry: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656]

Flow Cytometry: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656] - Staining of permeabilised Mouse peritoneal Macrophages cells with Rat anti Mouse CD68 Antibody (Clone FA-11) visualised with F(ab')2 Goat Anti Rat IgG:FITC (Mouse Adsorbed). Image from the standard format of this antibody.
Immunohistochemistry: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656]

Immunohistochemistry: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656]

Immunohistochemistry: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656] - Mouse spleen cryosection. Image from the standard format of this antibody.
Flow Cytometry: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656]

Flow Cytometry: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656]

Flow Cytometry: CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free [NBP2-80656] - An intracellular stain was performed on Raw264.7 cells with CD68/SR-D1 Antibody (FA-11) NBP2-33337B (blue) and a matched isotype control (orange). Both antibodies were directly conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals). Image from the standard format of this antibody.

Applications for CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Functional

reported in scientific literature (PMID 11085350)

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

2 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 uL of suggested dilution to label 10^6 cells in 100 uL. IHC requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD68/SR-D1

Human CD68, also known as GP110, LAMP4, Scavenger Receptor D1 (SR-D1) or macrosialin in mouse, encodes a 110-kD transmembrane glycoprotein that belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. Members of the LAMP family include LAMP-1, LAMP-2, dendritic cell (DC)-LAMP (aka CD208), and brain and dendritic cell-associated (BAD)-LAMP (aka LAMP-5). Unlike the two LAMP domains facing the lysosomal lumen in LAMP-1 and LAMP-2, CD68 has a single LAMP domain containing four cystines spaced 36-37 residues apart along with an N-terminal Mucin-like domain. The 354 amino acid (a.a.) human CD68 and 326 a.a. murine ortholog share 80.6% a.a. sequence identity (1).

CD68 is highly expressed in cells of the mononuclear phagocyte system such as macrophages, microglia, osteoclasts, and myeloid dendritic cells (DCs); and is expressed to a lesser extent in lymphoid cells (CD19+ B lymphocytes and CD4+ T lymphocytes), human umbilical cord mesenchymal stem cells (MSCs), fibroblasts, endothelial cells, multiple non-hematopoietic cancer cell lines, and human arterial intimal smooth muscle cells (SMCs). Expression has been also observed in diseased states for granulocytes and neutrophils, in particular basophils from myeloproliferative disorders and intestinal neutrophils from inflammatory bowel disease (IBD), respectively (1).

Although the function of CD68 has yet to be established, it has often been used as an immunohistochemistry (IHC) marker of inflammation and for granular cell tumors (GCTs). CD68+ tumor associated macrophages (TAMs) has been suggested to be a predictive marker for poor cancer prognosis, but a meta-analysis showed the presence of CD68 is not correlated with survival (2). In addition, a role in hepatic malaria infection has been reported based on the finding that peptide P39 binds CD68, considered a receptor for malaria sporozoite, and inhibits parasite entry into Kupffer cells. CD68 was deemed a member of the Scavenger receptor family due to its upregulation in macrophages following inflammatory stimuli, ability to bind modified LDL, phosphatidylserine, and apoptotic cells, as well as shuttling between the plasma membrane and endosomes. CD68 has been linked to atherogenesis based on binding and internalization of its ligand, oxLDL (1).

References

1. Chistiakov, DA, Killingsworth, MC, Myasoedova, VA. Orekhov AN, Bobryshev YV. (2017) CD68/macrosialin: not just a histochemical marker. Lab Invest. 97:4-13. PMID: 27869795

2. Troiano G, Caponio VCA, Adipietro I, Tepedino M, Santoro R, Laino L, Lo Russo L, Cirillo N, Lo Muzio L. (2019) Prognostic significance of CD68+ and CD163+ tumor associated macrophages in head and neck squamous cell carcinoma: A systematic review and meta-analysis. Oral Oncol. 93:66-75. PMID: 31109698.

Alternate Names

CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1

Gene Symbol

CD68

Additional CD68/SR-D1 Products

Product Documents for CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free

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Product Specific Notices for CD68/SR-D1 Antibody (FA-11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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