CD7 Antibody (4D4F8) - BSA Free

Novus Biologicals | Catalog # NBP2-37367

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D4F8

Format

BSA Free
View all CD7 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CD7 (AA: 72-175) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse CD7 Antibody (4D4F8) - BSA Free (NBP2-37367) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD7 Antibody (4D4F8) - BSA Free

Western Blot: CD7 Antibody (4D4F8)BSA Free [NBP2-37367]

Western Blot: CD7 Antibody (4D4F8)BSA Free [NBP2-37367]

Western Blot: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis using CD7 mAb against HEK293 (1) and CD7 (AA: 72-175)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: CD7 Antibody (4D4F8) - BSA Free [NBP2-37367]

Immunohistochemistry-Paraffin: CD7 Antibody (4D4F8) - BSA Free [NBP2-37367]

Immunohistochemistry-Paraffin: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis of lung cancer tissues using CD7 mouse mAb with DAB staining.
Flow Cytometry: CD7 Antibody (4D4F8) - BSA Free [NBP2-37367]

Flow Cytometry: CD7 Antibody (4D4F8) - BSA Free [NBP2-37367]

Flow Cytometry: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis of Jurkat cells using CD7 mouse mAb (green) and negative control (red).
Western Blot: CD7 Antibody (4D4F8)BSA Free [NBP2-37367]

Western Blot: CD7 Antibody (4D4F8)BSA Free [NBP2-37367]

Western Blot: CD7 Antibody (4D4F8) [NBP2-37367] - Analysis using CD7 mAb against human CD7 recombinant protein. (Expected MW is 36.6 kDa).
ELISA: CD7 Antibody (4D4F8) - BSA Free [NBP2-37367]

ELISA: CD7 Antibody (4D4F8) - BSA Free [NBP2-37367]

ELISA: CD7 Antibody (4D4F8) [NBP2-37367] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for CD7 Antibody (4D4F8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD7

CD7, also known as gp40, is a 40 kD type I transmembrane glycoprotein member of the immunoglobulin superfamily. It is found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion.

Alternate Names

CD7, LEU-9, TP41

Gene Symbol

CD7

Additional CD7 Products

Product Documents for CD7 Antibody (4D4F8) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD7 Antibody (4D4F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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