CD79A Antibody (HM57) - BSA Free

Novus Biologicals | Catalog # NB100-64347

Clone HM57 was used by HLDA to establish CD designation.
Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Avian - Chicken, Bovine, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HM57

Format

BSA Free
View all CD79A Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1

Reactivity Notes

Predicted cross-reactivities: Equine, Monkey, Guinea Pig, Goat, Fallow deer, Ferret, Dog, Bovine, American Bison, Porcine, Rabbit, Mouse, Rat, Red deer

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

NB100-64347 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin. Clone HM57 has been reported to work in western blotting applications.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD79A Antibody (HM57) - BSA Free

Immunohistochemistry: CD79A Antibody (HM57) - BSA Free [NB100-64347]

Immunohistochemistry: CD79A Antibody (HM57) - BSA Free [NB100-64347]

Immunohistochemistry: CD79A Antibody (HM57) [NB100-64347] - Paraffin-embedded alcohol fixed Rat Spleen tissue (20x). Antigen retrieval pH6. Dilution: 1:500 and ON incubation at 4C. This image was submitted via customer revies.
Flow Cytometry: CD79A Antibody (HM57) - BSA Free [NB100-64347]

Flow Cytometry: CD79A Antibody (HM57) - BSA Free [NB100-64347]

Flow Cytometry: CD79A Antibody (HM57) [NB100-64347] - Using the Allophycocyanin direct conjugate An intracellular stain was performed on Daudi cells with CD79A (HM 57) antibody NB100-64347APC (blue) and a matched isotype control NBP1-97005APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 0.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
Immunohistochemistry-Paraffin: CD79A Antibody (HM57) - BSA Free [NB100-64347]

Immunohistochemistry-Paraffin: CD79A Antibody (HM57) - BSA Free [NB100-64347]

Immunohistochemistry-Paraffin: CD79A Antibody (HM57) [NB100-64347] - Embedded human tonsil.

Applications for CD79A Antibody (HM57) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. For IHC: heat treated antigen retrieval using sodium citrate buffer pH 6.0 is recommended for this purpose. Although not tested this antibody may be useful in Western Blot. NB100-64347 has been used successfully in Immunofluorescence reported by a by a customer review.

Reviewed Applications

Read 6 reviews rated 3.5 using NB100-64347 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD79A

A disulfide-linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, is non-covalently associated with membrane bound immunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling.

Alternate Names

CD79A, MB-1

Entrez Gene IDs

973 (Human); 12518 (Mouse); 681236 (Rat)

Gene Symbol

CD79A

UniProt

P11912

Additional CD79A Products

Product Documents for CD79A Antibody (HM57) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD79A Antibody (HM57) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

B Cell Activation

Citations for CD79A Antibody (HM57) - BSA Free

Customer Reviews for CD79A Antibody (HM57) - BSA Free (6)

3.5 out of 5
6 Customer Ratings
5 Stars
17%
4 Stars
17%
3 Stars
67%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 6 reviews Showing All
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  • CD79A Antibody (HM57) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Tonsil
    Species: Human
    Verified Customer | Posted 10/13/2023
    CD79a NB100-64347 immunoreactivity in human tonsil. The antibody was diluted to 0.5ug/mL and was incubated with sections for 30min at room temperature. Secondary was Horse Anti-Mouse HRP polymer.
    Reacts with smooth muscle
    CD79A Antibody (HM57) - BSA Free NB100-64347
  • CD79A Antibody (HM57) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Skin
    Species: Equine
    Verified Customer | Posted 10/13/2023
    CD79a NB100-64347 immunoreactivity in horse skin (lymphoma). The antibody was diluted to 0.5ug/mL and was incubated with tissue section for 30min at room temperature. Secondary was Horse Anti-Mouse HRP polymer.
    Reacted strongly with smooth muscle as well as immune cells.
    CD79A Antibody (HM57) - BSA Free NB100-64347
  • CD79A Antibody (HM57) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Skin
    Species: Feline
    Verified Customer | Posted 10/13/2023
    CD79a NB100-64347 immunoreactivity in cat skin (lymphoma). Antibody was diluted to 0.5ug/mL and was left on tissue sections for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer.
    Reacted with muscle and nucleus. Attempts to limit this labeling resulted in a loss of suspected genuine immunoreactivity as well.
    CD79A Antibody (HM57) - BSA Free NB100-64347
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • CD79A Antibody (HM57) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: cynomolgus monkey lymph node and Lymph Node
    Species: Canine
    Verified Customer | Posted 10/13/2023
    CD79a NB100-64347 immunoreactivity in dog lymph node. Antibody was diluted to 0.5ug/mL and was left on tissue sections for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer.
    Reacts with smooth muscle. Attempts to dilute the antibody to eliminate muscle labeling resulted in a loss of signal in immune cells.
    CD79A Antibody (HM57) - BSA Free NB100-64347
  • CD79A Antibody (HM57)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Spleen tissue
    Species: Rat
    Verified Customer | Posted 02/17/2018
    IHC: Rat spleen tissue (20X). CD79a dilution 1:500 incubation ON at 4°C
    IHC performed on paraffin-embedded alcohol fixed tissue. Antigen retrieval pH6. Dilution: 1:500 and ON incubation at 4°C.
    CD79A Antibody (HM57) - BSA Free NB100-64347
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Dog Lymphoma
    Species: Other
    Verified Customer | Posted 08/15/2012
    CD79A Antibody (HM57) - BSA Free NB100-64347

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