CD81 Antibody (M38) - BSA Free

Novus Biologicals | Catalog # NBP1-44861

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Feline, Rabbit

Cited:

Human, Mouse, Flow

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot, Immunoprecipitation, Feline

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M38

Format

BSA Free
View all CD81 Primary Antibodies »

Product Specifications

Immunogen

MOLT-4, human T-ALL cell line (NM_004356.3).

Reactivity Notes

Cat (100%). Use in Mouse reported in scientific publication( PMID: 32573489).

Specificity

The antibody M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD81 Antibody (M38) - BSA Free

Knockout Validated: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Immunocytochemistry/ Immunofluorescence: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Immunocytochemistry/ Immunofluorescence: CD81 Antibody (M38) [NBP1-44861] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP1-44861) at 15 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.
Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Surface staining pattern of human peripheral blood stained using anti-human CD81 (M38) purified antibody (concentration in sample 4 ug/ml) GAM APC.
Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Surface staining of CD81 in human peripheral blood with anti-CD81 (M38) APC-CyTM7.
Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Human peripheral blood with anti-CD81 (M38) APC.
Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Human peripheral blood with anti-CD81 (M38) FITC.
Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Flow Cytometry: CD81 Antibody (M38) [PE] [NBP1-44861PE] - A cell surface stain was performed on THP-1 cells with CD81 antibody (M38) NBP1-44861 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE. Image using the PE form of this antibody.
Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) PE.
Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) PE.
Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) biotin, streptavidin-APC.
Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) - BSA Free [NBP1-44861]

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD81 (M38) purified antibody (concentration in sample 4 ug/ml) GAM APC.

Applications for CD81 Antibody (M38) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:50

Western Blot

1-2 ug/ml
Application Notes
Western blotting: positive control: Jurkat cells, non-reducing conditions. Clone M38 has been used for extracellular vesicle flow cytometry.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD81

CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells; e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.

Alternate Names

CD81, TAPA-1, TSPAN28

Gene Symbol

CD81

Additional CD81 Products

Product Documents for CD81 Antibody (M38) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD81 Antibody (M38) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD81 Antibody (M38) - BSA Free

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Protocols

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