CD9 Antibody (MEM-61) - BSA Free

Novus Biologicals | Catalog # NB500-327

Clone MEM-61 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, In vitro assay, CyTOF-ready

Cited:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vitro assay, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-61

Format

BSA Free
View all CD9 Primary Antibodies »

Product Specifications

Immunogen

Pre-B cell line NALM-6

Specificity

The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. HLDA VI; WS Code P P-15

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD9 Antibody (MEM-61) - BSA Free

Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) Alexa Fluor® 647.
Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of NALM-6 human pre-B cell leukemia cell line Total viable cells were used for analysis.
Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD9 (MEM-61) Dy161.
Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) biotin / streptavidin-APC.
Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) APC-CyTM7.
Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) Pacific BlueTM.
Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) APC.
Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) - BSA Free [NB500-327]

Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) PE.
CyTOF-ready: CD9 Antibody (MEM-61) [NB500-327] - Analysis of CD9 antibody on human tissue. Scale, 50um. Image from verified customer review.

Applications for CD9 Antibody (MEM-61) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

20 ug/ml

Western Blot

2-4 ug/ml
Application Notes
Western Blot -The antibody MEM-61 induces FcR-dependent platelet aggregation. This antibody is CyTOF ready.Use in In vitro reported in scientific literature (PMID: 28823976). Use in ELISA reported in secitific publication PMID: 32393780. Clone MEM-61 has been used for extracellular vesicle flow cytometry.

Reviewed Applications

Read 1 review rated 4 using NB500-327 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD9

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

Alternate Names

CD9, DRAP-27, MIC3, p24, TSPAN29

Gene Symbol

CD9

Additional CD9 Products

Product Documents for CD9 Antibody (MEM-61) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD9 Antibody (MEM-61) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD9 Antibody (MEM-61) - BSA Free

Customer Reviews for CD9 Antibody (MEM-61) - BSA Free (1)

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Customer Images

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  • CD9 Antibody (MEM-61)
    Name: Enkhtuya Radnaa
    Application: CyTOF
    Sample Tested: Human tissue origin
    Species: Human
    Verified Customer | Posted 01/04/2022
    Scale, 50um.
    CD9 Antibody (MEM-61) - BSA Free NB500-327

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