CD9 Antibody (SA35-08)

Novus Biologicals | Catalog # NBP2-67310

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Westen Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SA35-08 expressed in HEK293
View all CD9 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human CD9 aa 179-228 / 228. (SwissProt: P21926 Human; SwissProt: P40240 Mouse; SwissProt: P40241 Rat)

Epitope

cytoplasmic

Localization

Cell membrane, Membrane

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD9 Antibody (SA35-08)

Western Blot: CD9 Antibody (SA35-08) [NBP2-67310]

Western Blot: CD9 Antibody (SA35-08) [NBP2-67310]

Western Blot: CD9 Antibody (SA35-08) [NBP2-67310] - Exosome or whole cell lysates (WCL) sample from MDA-MB-231 cells was loaded with 10 ug/lane. 10% SDS-PAGE. CD9 Antibody (NBP2-67310) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: CD9 Antibody (SA35-08) [NBP2-67310]

Immunocytochemistry/ Immunofluorescence: CD9 Antibody (SA35-08) [NBP2-67310]

Immunocytochemistry/Immunofluorescence: CD9 Antibody (SA35-08) [NBP2-67310] - Staining CD9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m
Immunocytochemistry/ Immunofluorescence: CD9 Antibody (SA35-08) [NBP2-67310]

Immunocytochemistry/ Immunofluorescence: CD9 Antibody (SA35-08) [NBP2-67310]

Immunocytochemistry/Immunofluorescence: CD9 Antibody (SA35-08) [NBP2-67310] - Staining CD9 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Analysis of paraffin-embedded human kidney tissue using anti-CD9 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Analysis of paraffin-embedded human spleen tissue using anti-CD9 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Analysis of paraffin-embedded human tonsil tissue using anti-CD9 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Analysis of paraffin-embedded mouse brain tissue using anti-CD9 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Analysis of paraffin-embedded mouse spleen tissue using anti-CD9 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m
Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310]

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m
CD9 Antibody (SA35-08)

Western Blot: CD9 Antibody (SA35-08) [NBP2-67310] -

Western Blot: CD9 Antibody (SA35-08) [NBP2-67310] -Analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (ET1601-9) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: K-562 cell lysateLane 3: MCF7 cell lysateLane 4: HCT 116 cell lysateLane 5: HepG2 cell lysateLane 6: SK-MEL-28 cell lysateLane 7: A375 cell lysateLane 8: B16-F1 cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 25 kDaObserved band size: 23 kDaExposure time: 3 minutes 30 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for CD9 Antibody (SA35-08)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

Reviewed Applications

Read 1 review rated 5 using NBP2-67310 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD9

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behavior in peripheral nerve.

Alternate Names

CD9, DRAP-27, MIC3, p24, TSPAN29

Gene Symbol

CD9

Additional CD9 Products

Product Documents for CD9 Antibody (SA35-08)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD9 Antibody (SA35-08)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD9 Antibody (SA35-08)

Customer Reviews for CD9 Antibody (SA35-08) (1)

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Customer Images

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  • CD9 Antibody (SA35-08)
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: MDA MB 231 whole cell lysates and conditioned medium
    Species: Human
    Verified Customer | Posted 10/23/2022
    Western Blot: Exosome or whole cell lysates (WCL) sample from MDA-MB-231 cells was loaded with 10 ug/lane. 10% SDS-PAGE. CD9 Antibody (NBP2-67310) was used for primary antibody: 1:1000, 4℃, overnight.
    CD9 Antibody (SA35-08) NBP2-67310

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Protocols

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