CD90/Thy1 Antibody (5E10) [PerCP/Cy5.5]

Novus Biologicals | Catalog # NBP1-42847

Clone 5E10 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PerCP/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5E10
View all CD90/Thy1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD90.

Marker

Mesenchymal Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD90/Thy1 Antibody (5E10) [PerCP/Cy5.5]

Flow Cytometry: CD90/Thy1 Antibody (5E10) [PerCP/Cy5.5] [NBP1-42847] - Staining of the HEL cell line with Mouse IgG1 kappa Isotype Control PerCP-Cy5.5 (open histogram) or Anti-Human CD90 (Thy-1) PerCP-Cy5.5 (filled histogram). Total viable cells were used for analysis.

Applications for CD90/Thy1 Antibody (5E10) [PerCP/Cy5.5]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
For Flow Cytometry: Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Reviewed Applications

Read 1 review rated 3 using NBP1-42847 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin and 0.2% BSA

Preservative

0.09% Sodium Azide

Concentration

5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD90/Thy1

The 5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Alternate Names

CD90, Thy1

Gene Symbol

THY1

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody (5E10) [PerCP/Cy5.5]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD90/Thy1 Antibody (5E10) [PerCP/Cy5.5]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD90/Thy1 Antibody (5E10) [PerCP/Cy5.5] (1)

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Customer Images

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  • CD90/Thy1 Antibody (5E10)
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Whole blood cells
    Species: Feline
    Verified Customer | Posted 11/17/2017
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    CD90/Thy1 Antibody (5E10) [PerCP/Cy5.5] NBP1-42847

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FAQs for CD90/Thy1 Antibody (5E10) [PerCP/Cy5.5]

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    • Q: We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
      A: I would recommend one of theses CD90 antibodies, as these have all been validated for use with dog/canine samples.
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