CD90/Thy1 Antibody (5E10) - BSA Free

Novus Biologicals | Catalog # NBP1-43379

Clone 5E10 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation

Cited:

Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5E10

Format

BSA Free
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Product Specifications

Immunogen

The immunogen for this antibody was CD90.

Marker

Mesenchymal Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD90/Thy1 Antibody (5E10) - BSA Free

Flow Cytometry: CD90/Thy1 Antibody (5E10) - BSA Free [NBP1-43379]

Flow Cytometry: CD90/Thy1 Antibody (5E10) - BSA Free [NBP1-43379]

Flow Cytometry: CD90/Thy1 Antibody (5E10) [NBP1-43379] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-43379. Staining of the HEL cell line with Mouse IgG1 kappa Isotype Control PerCP-Cy5.5 (open histogram) or Anti-Human CD90 (Thy-1) PerCP-Cy5.5 (filled histogram). Total viable cells were used for analysis.
Flow Cytometry: CD90/Thy1 Antibody (5E10) - BSA Free [NBP1-43379]

Flow Cytometry: CD90/Thy1 Antibody (5E10) - BSA Free [NBP1-43379]

Flow Cytometry: CD90/Thy1 Antibody (5E10) [NBP1-43379] - Staining of human erythroleukemia (HEL) cell line with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (NBP1-43319) (open histogram) or 0.25 ug of Anti-Human CD90 (Thy-1) Purified (filled histogram) followed by Anti-Mouse IgG FITC. Total viable cells were used for analysis.

Applications for CD90/Thy1 Antibody (5E10) - BSA Free

Application
Recommended Usage

Flow Cytometry

< = 0.5 ug/10^6 cells in 100 ul

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For Flow Cytometry: Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD90/Thy1

The 5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Alternate Names

CD90, Thy1

Gene Symbol

THY1

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody (5E10) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD90/Thy1 Antibody (5E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD90/Thy1 Antibody (5E10) - BSA Free

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Protocols

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FAQs for CD90/Thy1 Antibody (5E10) - BSA Free

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  • Q: We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A: I would recommend one of theses CD90 antibodies, as these have all been validated for use with dog/canine samples.

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