CDC42EP2 Antibody (2H7) - Azide and BSA Free

Novus Biologicals | Catalog # H00010435-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2H7

Format

Azide and BSA Free
View all CDC42EP2 Primary Antibodies »

Product Specifications

Immunogen

CDC42EP2 (NP_006770, 102 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLLKNAISLPVIGGPQALTLPTAQAPPKPPRLHLETPQPSPQEGGSVDIWRIPETGSPNSGLTPESGAEEPFLSNASSLLSLHVDLGPSILDDVLQIMDQDLDSMQIPT

Reactivity Notes

Human. Other species not tested.

Specificity

CDC42EP2 - CDC42 effector protein (Rho GTPase binding) 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CDC42EP2 Antibody (2H7) - Azide and BSA Free

Western Blot: CDC42EP2 Antibody (2H7) [H00010435-M01]

Western Blot: CDC42EP2 Antibody (2H7) [H00010435-M01]

Western Blot: CDC42EP2 Antibody (2H7) [H00010435-M01] - Analysis of CDC42EP2 expression in transfected 293T cell line by CDC42EP2 monoclonal antibody (M01), clone 2H7.Lane 1: CDC42EP2 transfected lysate(22.5 KDa).Lane 2: Non-transfected lysate.

Applications for CDC42EP2 Antibody (2H7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CDC42EP2

CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. Coexpression of this protein with dominant negative mutant CDC42 protein in fibroblast was found to induce pseudopodia formation, which suggested a role of this protein in actin filament assembly and cell shape control.

Alternate Names

BORG1CEP2Binder of Rho GTPases 1, CDC42 effector protein (Rho GTPase binding) 2, cdc42 effector protein 2, CRIB-containing BOGR1 protein

Entrez Gene IDs

10435 (Human)

Gene Symbol

CDC42EP2

OMIM

606132 (Human)

UniProt

NP_006770 (Human)

Additional CDC42EP2 Products

Product Documents for CDC42EP2 Antibody (2H7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CDC42EP2 Antibody (2H7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CDC42EP2 Antibody (2H7) - Azide and BSA Free

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Protocols

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FAQs

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