CDC5L Antibody (1E3) - BSA Free

Novus Biologicals | Catalog # NBP3-26668

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1E3 expressed in HEK293

Format

BSA Free
View all CDC5L Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human CDC5L [UniProt Q99459]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC5L Antibody (1E3) - BSA Free

CDC5L Antibody (1E3)

Immunoprecipitation: CDC5L Antibody (1E3) [NBP3-26668] -

Immunoprecipitation: CDC5L Antibody (1E3) [NBP3-26668] - Immunoprecipitating CDC5L in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26668 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26668 (3ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (20ug).
CDC5L Antibody (1E3)

Immunohistochemistry: CDC5L Antibody (1E3) [NBP3-26668] -

Immunohistochemistry: CDC5L Antibody (1E3) [NBP3-26668] - Image of CDC5L Antibody (1E3) diluted at 1:126.5 and staining in paraffin-embedded human appendix tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CDC5L Antibody (1E3)

Immunohistochemistry: CDC5L Antibody (1E3) [NBP3-26668] -

Immunohistochemistry: CDC5L Antibody (1E3) [NBP3-26668] - Image of CDC5L Antibody (1E3) diluted at 1:126.5 and staining in paraffin-embedded human appendix tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CDC5L Antibody (1E3)

Western Blot: CDC5L Antibody (1E3) [NBP3-26668] -

Western Blot: CDC5L Antibody (1E3) [NBP3-26668] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate.
All lanes: CDC5L Antibody at 1.3ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 93 KDa
Observed band size: 100 KDa
CDC5L Antibody (1E3)

Immunocytochemistry/Immunofluorescence: CDC5L Antibody (1E3) [NBP3-26668] -

Immunocytochemistry/Immunofluorescence: CDC5L Antibody (1E3) [NBP3-26668] - Staining of A549 cells with CDC5L Antibody (1E3) at 1:42, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for CDC5L Antibody (1E3) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CDC5L

CDC5L is encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq]

Alternate Names

CDC5, CDC5 (cell division cycle 5, S. pombe, homolog)-like, CDC5 cell division cycle 5-like (S. pombe), Cdc5-like protein, Cdc5-related protein, CEF1, cell division cycle 5-like protein, dJ319D22.1, dJ319D22.1 (CDC5-like protein), hCDC5, KIAA0432, PCDC5RPCDC5-LIKE, Pombe cdc5-related protein

Gene Symbol

CDC5L

Additional CDC5L Products

Product Documents for CDC5L Antibody (1E3) - BSA Free

Certificate of Analysis

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Product Specific Notices for CDC5L Antibody (1E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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