CDK2 [p Tyr15] Antibody (2C4) - BSA Free

Novus Biologicals | Catalog # NBP3-26428

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2C4 expressed in HEK293

Format

BSA Free
View all CDK2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human CDK2 [p Tyr15] [UniProt P24941]

Modification

p Tyr 15

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CDK2 [p Tyr15] Antibody (2C4) - BSA Free (NBP3-26428) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CDK2 [p Tyr15] Antibody (2C4) - BSA Free

CDK2 [p Tyr15] Antibody (2C4)

Immunoprecipitation: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] -

Immunoprecipitation: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Immunoprecipitating CDK2 [p Tyr15] in Hela whole cell lysate treated with Pervanadate.
Lane 1: Rabbit control IgG (1ug)instead of NBP3-26428 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26428 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).
Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).
CDK2 [p Tyr15] Antibody (2C4)

Western Blot: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] -

Western Blot: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate (treated with Pervanadate or not).
All lanes: CDK2 [p Tyr15] Antibody at 0.8ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 34 KDa
Observed band size: 34 KDa
CDK2 [p Tyr15] Antibody (2C4)

Immunocytochemistry/Immunofluorescence: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] -

Immunocytochemistry/Immunofluorescence: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Analysis of CDK2 [p Tyr15] Antibody (2C4) diluted at 1:80 and staining in Hela cells (treated with Pervanadate). The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Applications for CDK2 [p Tyr15] Antibody (2C4) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CDK2

The protein encoded by the Cdk2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highlysimilar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of thecyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycleG1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex includingcyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its proteinphosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have beenreported. (provided by RefSeq)

Long Name

Cyclin-dependent Kinase 2

Alternate Names

cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)

Gene Symbol

CDK2

Additional CDK2 Products

Product Documents for CDK2 [p Tyr15] Antibody (2C4) - BSA Free

Certificate of Analysis

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Product Specific Notices for CDK2 [p Tyr15] Antibody (2C4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Intracellular Kinases

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Protocols

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FAQs

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail