Cdk5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-38178

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CDK5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human Cdk5 (NP_004926.1).

Sequence:
PDVLFGAKLYSTSIDMWSAGCIFAELANAGRPLFPGNDVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYFSDF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Cdk5 Antibody - BSA Free (NBP3-38178) is a polyclonal antibody validated for use in WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Cdk5 Antibody - BSA Free

Cdk5 Antibody

Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody [NBP3-38178] -

Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody [NBP3-38178] - Immunofluorescence analysis of U2OS cells using [KO Validated] Cdk5 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Cdk5 Antibody

Western Blot: Cdk5 Antibody [NBP3-38178] -

Western Blot: Cdk5 Antibody [NBP3-38178] - Western blot analysis of various lysates using [KO Validated] Cdk5 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Cdk5 Antibody

Western Blot: Cdk5 Antibody [NBP3-38178] -

Western Blot: Cdk5 Antibody [NBP3-38178] - Western blot analysis of various lysates using [KO Validated] Cdk5 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Cdk5 Antibody

Immunoprecipitation: Cdk5 Antibody [NBP3-38178] -

Immunoprecipitation: Cdk5 Antibody [NBP3-38178] - Immunoprecipitation analysis of 900 ug extracts of A-549 cells using 3 ug Cdk5 antibody. Western blot was performed from the immunoprecipitate using Cdk5 antibody at a dilution of 1:1000.
Cdk5 Antibody

Western Blot: Cdk5 Antibody [NBP3-38178] -

Western Blot: Cdk5 Antibody [NBP3-38178] - Western Blot analysis of lysates from wild type (WT) and Cdk5 knockout (KO) 293T cells, using [KO Validated] Cdk5 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Applications for Cdk5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Immunoprecipitation

0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDK5

Long Name

Cyclin-dependent Kinase 5

Alternate Names

CDKN5, LIS7, PSSALRE

Gene Symbol

CDK5

Additional CDK5 Products

Product Documents for Cdk5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cdk5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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