Cdk6 Antibody (8H4) - Azide and BSA Free

Novus Biologicals | Catalog # H00001021-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 8H4

Format

Azide and BSA Free
View all Cdk6 Primary Antibodies »

Product Specifications

Immunogen

CDK6 (NP_001250, 3 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KDGLCRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTGEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFE

Specificity

CDK6 - cyclin-dependent kinase 6

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cdk6 Antibody (8H4) - Azide and BSA Free

Western Blot: Cdk6 Antibody (8H4) [H00001021-M01]

Western Blot: Cdk6 Antibody (8H4) [H00001021-M01]

Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.Lane 1: CDK6 transfected lysate(36.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Cdk6 Antibody (8H4) [H00001021-M01]

Immunocytochemistry/ Immunofluorescence: Cdk6 Antibody (8H4) [H00001021-M01]

Immunocytochemistry/Immunofluorescence: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of monoclonal antibody to CDK6 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Cdk6 Antibody (8H4) [H00001021-M01]

Immunohistochemistry-Paraffin: Cdk6 Antibody (8H4) [H00001021-M01]

Immunohistochemistry-Paraffin: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of monoclonal antibody to CDK6 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Western Blot: Cdk6 Antibody (8H4) [H00001021-M01]

Western Blot: Cdk6 Antibody (8H4) [H00001021-M01]

Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - CDK6 monoclonal antibody (M01), clone 8H4. Analysis of CDK6 expression in PC-12.
Western Blot: Cdk6 Antibody (8H4) [H00001021-M01]

Western Blot: Cdk6 Antibody (8H4) [H00001021-M01]

Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - CDK6 monoclonal antibody (M01), clone 8H4 Analysis of CDK6 expression in Jurkat.
Proximity Ligation Assay: Cdk6 Antibody (8H4) [H00001021-M01]

Proximity Ligation Assay: Cdk6 Antibody (8H4) [H00001021-M01]

Proximity Ligation Assay: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of protein-protein interactions between CDK2 and CDK6. HeLa cells were stained with anti-CDK2 rabbit purified polyclonal 1:1200 and anti-CDK6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
ELISA: Cdk6 Antibody (8H4) [H00001021-M01]

ELISA: Cdk6 Antibody (8H4) [H00001021-M01]

ELISA: Cdk6 Antibody (8H4) [H00001021-M01] - Detection limit for recombinant GST tagged CDK6 is 0.3 ng/ml as a capture antibody.

Applications for Cdk6 Antibody (8H4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cdk6

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.

Long Name

Cyclin-dependent kinase 6

Alternate Names

CDKN6

Entrez Gene IDs

1021 (Human)

Gene Symbol

CDK6

OMIM

603368 (Human)

UniProt

NP_001250 (Human)

Additional Cdk6 Products

Product Documents for Cdk6 Antibody (8H4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cdk6 Antibody (8H4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cdk6 Antibody (8H4) - Azide and BSA Free

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Protocols

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FAQs

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