![Western Blot: CDX2 Antibody [NBP2-24583]](https://beta-resources.rndsystems.com/images/products/CDX2-Antibody-Western-Blot-NBP2-24583-img0003.jpg "Western Blot: CDX2 Antibody [NBP2-24583]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Equine, Opossum, Primate
Predicted:
Monkey (93%), Rhesus Macaque (93%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A portion of amino acids 1-60 of human CDX2 /CDX3 was used as the immunogen for the antibody.
Reactivity Notes
The amino acid sequence used as immunogen for the GATA-4 antibody is 93% homologous in Rhesus Macaque, 87% homologous in Rat, 82% in Mouse, and 81% homologous in Canine.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CDX2 Antibody - BSA Free (NBP2-24583) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CDX2 Antibody - BSA Free
Western Blot: CDX2 Antibody [NBP2-24583]
Western Blot: CDX2 Antibody [NBP2-24583] - Total protein from human HeLa, HepG2, CaCo2 and HCT116, mouse 3T3 and Neuro2A and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CDX2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.![Immunohistochemistry-Paraffin: CDX2 Antibody [NBP2-24583]](https://beta-resources.rndsystems.com/images/products/CDX2-Antibody-Immunohistochemistry-Paraffin-NBP2-24583-img0002.jpg "Immunohistochemistry-Paraffin: CDX2 Antibody [NBP2-24583]")
Immunohistochemistry-Paraffin: CDX2 Antibody [NBP2-24583]
Immunohistochemistry-Paraffin: CDX2 Antibody [NBP2-24583] - Formalin-fixed, paraffin-embedded human spleen probed with CDX2 / CDX3 antibody at 5 ug/ml.![Western Blot: CDX2 Antibody [NBP2-24583]](https://beta-resources.rndsystems.com/images/products/CDX2-Antibody-Western-Blot-NBP2-24583-img0001.jpg "Western Blot: CDX2 Antibody [NBP2-24583]")
Western Blot: CDX2 Antibody [NBP2-24583]
Western Blot: CDX2 Antibody [NBP2-24583] - Analysis of CDX3/CDX2 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Applications for CDX2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1-3 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CDX2
Long Name
Caudal-type Homeobox Transcription Factor 2
Alternate Names
CDX3
Gene Symbol
CDX2
UniProt
Additional CDX2 Products
Product Documents for CDX2 Antibody - BSA Free
Product Specific Notices for CDX2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
