CEACAM1/CD66a Antibody (BLR032F)
Novus Biologicals | Catalog # NBP2-76405
Recombinant Monoclonal Antibody
![Western Blot: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]](https://resources.rndsystems.com/images/products/CEACAM1-CD66a-Antibody-BLR032F-Western-Blot-NBP2-76405-img0003.jpg "Western Blot: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # BLR032F
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Product Specifications
Immunogen
Recombinant protein representing the extracellular domain of human CEACAM1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CEACAM1/CD66a Antibody (BLR032F)
Western Blot: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]
Western Blot: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405] - Detection of human CEACAM1 by WB of UACC-257, SK-MEL-28, Hep-G2, SUP-M2, HeLa, HEK293T, Jurkat, RAW 264.7, and TCMK-1 lysate. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] (NBP2-76405). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-Actin.![Immunocytochemistry: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]](https://resources.rndsystems.com/images/products/CEACAM1-CD66a-Antibody-BLR032F-Immunocytochemistry-NBP2-76405-img0002.jpg "Immunocytochemistry: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]")
Immunocytochemistry: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]
Immunocytochemistry: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405] - Detection of human CEACAM1 in FFPE HT-29 cells by ICC. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] (NBP2-76405). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.![Immunohistochemistry-Paraffin: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]](https://resources.rndsystems.com/images/products/CEACAM1-CD66a-Antibody-BLR032F-Immunocytochemistry-NBP2-76405-img0001.jpg "Immunohistochemistry-Paraffin: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]")
Immunohistochemistry-Paraffin: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405]
Immunohistochemistry-Paraffin: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405] - Detection of human CEACAM1 in FFPE colon carcinoma by ICC. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] (NBP2-76405). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405] - Detection of human CEACAM1 by WB of immunoprecipitates from UACC-257 lysate. Antibodies: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] NBP2-76405 and rabbit anti-CEACAM1 recombinant monoclonal.
Applications for CEACAM1/CD66a Antibody (BLR032F)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 to 1:500
Immunohistochemistry
1:100 - 1:500
Immunoprecipitation
20 ul/1 mg lysate
Western Blot
1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: CEACAM-1/CD66a
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 1
Alternate Names
BGP1, Biliary Glycoprotein 1, CD66a, Cea-1, CEACAM1, Hv-1, Hv-2, MHVR
Gene Symbol
CEACAM1
Additional CEACAM-1/CD66a Products
Product Documents for CEACAM1/CD66a Antibody (BLR032F)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CEACAM1/CD66a Antibody (BLR032F)
This Product sold under License from Bethyl Laboratories, Inc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
