CEBP alpha Antibody (HL1747) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25386

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Mouse (93%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1747 expressed in HEK293

Format

Azide and BSA Free
View all CEBP alpha Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CEBP alpha. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CEBP alpha Antibody (HL1747) - Azide and BSA Free

CEBP alpha Antibody (HL1747) - Azide and BSA Free

Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] -

Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
CEBP alpha Antibody (HL1747) - Azide and BSA Free

Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] -

Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
CEBP alpha Antibody (HL1747) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] -

Immunocytochemistry/Immunofluorescence: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - C/EBP alpha antibody [HL1747] detects C/EBP alpha protein at nucleus by immunofluorescent analysis. Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min. Green: C/EBP alpha stained by C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for CEBP alpha Antibody (HL1747) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEBP alpha

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). C/EBP alpha (2) can form heterodimers with related protein C/EBP beta and gamma. C/EBP alpha directly interacts with CDK2and CDK4 and arrests cell proliferation by inhibiting these kinases (3). C/EBP alpha has been shown to bind to the promoter and modulate the expression of leptin, a protein that plays an important role in body weight homeostasis (4). C/EBP alpha also has prominent antimitotic activity and may play a role of a tumor suppressor.

Long Name

CCAAT/enhancer-Binding Protein alpha

Alternate Names

C/EBP-alpha, CEBPA

Gene Symbol

CEBPA

Additional CEBP alpha Products

Product Documents for CEBP alpha Antibody (HL1747) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CEBP alpha Antibody (HL1747) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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