CENPM Antibody (4C12-2C8) - Azide and BSA Free

Novus Biologicals | Catalog # H00079019-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4C12-2C8

Format

Azide and BSA Free
View all CENPM Primary Antibodies »

Product Specifications

Immunogen

C22orf18 (AAH00705, 1 a.a. ~ 180 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL

Localization

Cytoplasmic and Nuclear. Note: Nuclear in non confluent cells and cytoplasmic in confluent or dividing cells. Localizes in the kinetochore domain of centromeres.

Specificity

C22orf18 - chromosome 22 open reading frame 18

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CENPM Antibody (4C12-2C8) - Azide and BSA Free

Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01]

Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01]

Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01] - C22orf18 monoclonal antibody (M01), clone 4C12-2C8 Analysis of C22orf18 expression in K-562.
Immunocytochemistry/ Immunofluorescence: CENPM Antibody (4C12-2C8) [H00079019-M01]

Immunocytochemistry/ Immunofluorescence: CENPM Antibody (4C12-2C8) [H00079019-M01]

Immunocytochemistry/Immunofluorescence: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of monoclonal antibody to MGC861 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01]

Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01]

Western Blot: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody (M01), clone 4C12-2C8.Lane 1: CENPM transfected lysate (Predicted MW: 19.7 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: CENPM Antibody (4C12-2C8) [H00079019-M01]

Immunoprecipitation: CENPM Antibody (4C12-2C8) [H00079019-M01]

Immunoprecipitation: CENPM Antibody (4C12-2C8) [H00079019-M01] - Analysis of CENPM transfected lysate using anti-CENPM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CENPM MaxPab rabbit polyclonal antibody.
ELISA: CENPM Antibody (4C12-2C8) [H00079019-M01]

ELISA: CENPM Antibody (4C12-2C8) [H00079019-M01]

ELISA: CENPM Antibody (4C12-2C8) [H00079019-M01] - Detection limit for recombinant GST tagged C22orf18 is approximately 0.03ng/ml as a capture antibody.

Applications for CENPM Antibody (4C12-2C8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CENPM

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM]

Alternate Names

bK250D10.2, C22orf18PANE1MGC861, CENP-Mchromosome 22 open reading frame 18, centromere protein M, ICEN39, Interphase centromere complex protein 39, Pane1, proliferation associated nuclear element 1, Proliferation-associated nuclear element protein 1

Entrez Gene IDs

79019 (Human)

Gene Symbol

CENPM

UniProt

AAH00705 (Human)

Additional CENPM Products

Product Documents for CENPM Antibody (4C12-2C8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CENPM Antibody (4C12-2C8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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