Centaurin alpha 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88097

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Centaurin alpha 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PKQTEGFRKRWFTMDDRRLMYFKDPLDAFARGEVFIGSKESGYTVLHGFPPSTQGHHWPHGITIVTPDRKFLFACETESDQREWVAAFQKAVDRPMLPQEYAVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Centaurin alpha 1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Centaurin alpha 1 Antibody [NBP1-88097]

Immunocytochemistry/ Immunofluorescence: Centaurin alpha 1 Antibody [NBP1-88097]

Immunocytochemistry/Immunofluorescence: Centaurin alpha 1 Antibody [NBP1-88097] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097]

Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097]

Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097]

Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097]

Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097] - Staining of human Cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097]

Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097]

Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097] - Staining of human Cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097]

Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097]

Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097] - Staining of human Stomach shows strong cytoplasmic positivity in glandular cells.
Centaurin alpha 1 Antibody - BSA Free Western Blot: Centaurin alpha 1 Antibody - BSA Free [NBP1-88097]

Western Blot: Centaurin alpha 1 Antibody - BSA Free [NBP1-88097]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4

Applications for Centaurin alpha 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Centaurin alpha 1

The centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.

Alternate Names

arf-GAP with dual PH domain-containing protein 1, ArfGAP with dual PH domains 1, CENTA1centaurin-alpha-1, centaurin, alpha 1, centaurin-alpha, Centaurin-alpha-1, Cnt-a1, GCS1L, p42IP4, Putative MAPK-activating protein PM25

Gene Symbol

ADAP1

Additional Centaurin alpha 1 Products

Product Documents for Centaurin alpha 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Centaurin alpha 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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