CEP290 Antibody

Novus Biologicals | Catalog # NBP1-52132

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Immunofluorescence, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CEP290 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RNSKHLKQQQYRAEN corresponding to internal region according to NP_079390.3.

Epitope

C-RNSKHLKQQQYRAEN

Specificity

Despite similarity of the immunizing peptide with the mouse protein, this antibody was demonstrated to weak for mouse Cep290.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CEP290 Antibody

Immunocytochemistry/ Immunofluorescence: CEP290 Antibody [NBP1-52132]

Immunocytochemistry/ Immunofluorescence: CEP290 Antibody [NBP1-52132]

Immunocytochemistry/Immunofluorescence: CEP290 Antibody [NBP1-52132] - Overnight staining with antibody at 2.5 ug/mL of cell lines OPCT-1 (A) and MDA468 (B) with Alexa Fluor 568 (red) and nuclear counter staining with DAPI (blue), provided by Dr S, McArdle, and obtained by Sumanjeet Malhi, John van Geest Research Centre, Nottingham, UK.
Immunohistochemistry-Paraffin: CEP290 Antibody [NBP1-52132]

Immunohistochemistry-Paraffin: CEP290 Antibody [NBP1-52132]

Immunohistochemistry-Paraffin: CEP290 Antibody [NBP1-52132] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
Immunohistochemistry-Paraffin: CEP290 Antibody [NBP1-52132]

Immunohistochemistry-Paraffin: CEP290 Antibody [NBP1-52132]

Immunohistochemistry-Paraffin: CEP290 Antibody [NBP1-52132] - Staining of paraffin embedded Human Prostate with antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for CEP290 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2 - 3 ug/mL

Immunohistochemistry-Paraffin

5 - 7 ug/mL

Peptide ELISA

Detection limit 1:128000
Application Notes
ICC/IF: Formaldehyde-fixed cell lines derived from prostate cancer and from breast cancer show typical centrosomal staining.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CEP290

CEP290 activates ATF4 mediated transcription and is required for the correct localization of ciliary and phototransduction proteins in retinal photoreceptor cells; may play a role in ciliary transport processes. CEP290 is ubiquitously expressed; strongly in placenta and weakly in brain. There are two named isoforms. Defects in CEP290 are a cause of Joubert syndrome type 5 (JBTS5) [MIM:610188], Senior-Loken syndrome type 6 (SLSN6) [MIM:610189], Leber congenital amaurosis type 10 (LCA10) [MIM:611755] and Meckel syndrome type 4 (MKS4) [MIM:611134]. Antibodies against CEP290 are present in sera from patients with cutaneous T cell lymphomas, but not in the healthy control population.

Alternate Names

BBS14Bardet-Biedl syndrome 14 protein, Cancer/testis antigen 87, centrosomal protein 290kDa, Cep290, CT87JBTS6, FLJ13615, JBTS5CTCL tumor antigen se2-2, KIAA0373FLJ21979, LCA10monoclonal 3H11 antigen, MKS4, Nephrocystin-6, NPHP6centrosomal protein of 290 kDa, POC3, POC3 centriolar protein homolog, prostate cancer antigen T21, rd16,3H11AG, SLSN6nephrocytsin-6,3H11Ag, Tumor antigen se2-2

Entrez Gene IDs

80184 (Human); 216274 (Mouse)

Gene Symbol

CEP290

UniProt

NP_079390.3 (Human)

Additional CEP290 Products

Product Documents for CEP290 Antibody

Certificate of Analysis

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Product Specific Notices for CEP290 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CEP290 Antibody

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Protocols

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