CGI-16 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35548

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CGI-16 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-250 of human CGI-16 (NP_001028755.2).

Sequence:
LTQGPQNPKKQGIFHIHEVRDKLREIVGASTNWRDHVKAMEERKLLHSFLAKSQDGLPPRRMKDSYIEVLLPLGSEPELREKYLTVQNTVRFGRILEDLDSLGVLICYMHNKIHSAKMSPLSIVTALVDKIDMCKKSLSPEQDIKFSGHVSWVGKTSMEVKMQMFQLHGDEFCPVLDATFVMVARDSENKGPAFVNPLIPESPEEEELFRQGELNKGRRIAFSSTSLLK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CGI-16 Antibody - BSA Free

CGI-16 Antibody

Immunocytochemistry/ Immunofluorescence: CGI-16 Antibody [NBP3-35548] -

Immunocytochemistry/ Immunofluorescence: CGI-16 Antibody [NBP3-35548] - Immunofluorescence analysis of A431 cells using CGI-16 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
CGI-16 Antibody

Western Blot: CGI-16 Antibody [NBP3-35548] -

Western Blot: CGI-16 Antibody [NBP3-35548] - Western blot analysis of various lysates using CGI-16 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Applications for CGI-16 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:200 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CGI-16

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fattyacid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs

Alternate Names

ACATE2, Acyl-CoA thioester hydrolase 9, acyl-CoA thioesterase 9acyl-Coenzyme A thioesterase 2, mitochondrial, acyl-coenzyme A thioesterase 9, mitochondrial, CGI-16, EC 3.1.2, EC 3.1.2.-, EC 3.1.2.15, mitochondrial Acyl-CoA Thioesterase, MT-ACT48

Gene Symbol

ACOT9

Additional CGI-16 Products

Product Documents for CGI-16 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CGI-16 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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