CHD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-14478

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CHD1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: QESQQNSDQNSNLNPHVIRNPDVERLKENTNHDDSSRDSYSSDRHLTQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHRDWDHYKQDSRYYSDREKHRKLDDHRSRDHRSNLEGSL

Reactivity Notes

Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHD1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CHD1 Antibody [NBP2-14478]

Immunocytochemistry/ Immunofluorescence: CHD1 Antibody [NBP2-14478]

Immunocytochemistry/Immunofluorescence: CHD1 Antibody [NBP2-14478] - Staining of human cell line RH-30 shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478]

Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478]

Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining in human testis and skeletal muscle tissues.. Corresponding CHD1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478]

Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478]

Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human placenta shows moderate to strong nuclear positivity.
Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478]

Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478]

Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478]

Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478]

Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478]

Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478]

Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
CHD1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: CHD1 Antibody - BSA Free [NBP2-14478]

Chromatin Immunoprecipitation-exo-Seq: CHD1 Antibody - BSA Free [NBP2-14478]

ChIP-Exo-Seq composite graph for Anti-CHD1 (NBP2-14478) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for CHD1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHD1

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

Long Name

Chromodomain Helicase DNA Binding Protein 1

Alternate Names

ATP-dependent helicase CHD1, CHD-1, chromodomain helicase DNA binding protein 1, chromodomain-helicase-DNA-binding protein 1, DKFZp686E2337, EC 3.6.1, EC 3.6.4.12

Gene Symbol

CHD1

Additional CHD1 Products

Product Documents for CHD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CHD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CHD1 Antibody - BSA Free

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Protocols

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