Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CHD7 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to an N-terminal region of human CHD7 (within residues 25-200). [Swiss-Prot Q9P2D1]

Reactivity Notes

Human and mouse. Immunogen has 85% identity to chicken Chd7.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHD7 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CHD7 Antibody - BSA Free [NBP1-77393]

Immunocytochemistry/ Immunofluorescence: CHD7 Antibody - BSA Free [NBP1-77393]

Immunocytochemistry/Immunofluorescence: Chd7 Antibody [NBP1-77393] - Antibody was tested in NIH/3T3 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: CHD7 Antibody - BSA Free [NBP1-77393]

Immunohistochemistry: CHD7 Antibody - BSA Free [NBP1-77393]

Immunohistochemistry: Chd7 Antibody [NBP1-77393] - IHC analysis of CHD7 in mouse intestine using DAB with hematoxylin counterstain.

Applications for CHD7 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:250

Immunohistochemistry-Paraffin

1:250

Western Blot

1:2500
Application Notes
This CHD7 antibody is useful for IHC-P, ICC/IF and Western blot where a band is seen ~330 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-77393 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHD7

CHARGE syndrome (coloboma, heart defects, atresia of the choanae, retarded growth and development, genital hypoplasia, ear anomalies and deafness) is a congenital malformation syndrome caused by mutations in the CHD7 (chromodomain helicase DNA-binding protein) gene in approximately 2/3 of cases. In Kallmann syndrome, a similar proportion of affected individuals also have mutated CHD7. These mutations probably affect neurogenerative anomalies and maturation events through SOX2 interaction. Expression patterns of CHD7 in combination with SOX2 evaluation can provide some insight into molecular causes of CHARGE and Kallmann syndromes.

Long Name

Chromodomain Helicase DNA Binding Protein 7

Alternate Names

ATP-dependent helicase CHD7, CHD-7, chromodomain helicase DNA binding protein 7, chromodomain helicase DNA binding protein 7 isoform CRA_e, chromodomain-helicase-DNA-binding protein 7, EC 3.6.1, EC 3.6.4.12, FLJ20357, FLJ20361, IS3, KIAA1416KAL5

Entrez Gene IDs

55636 (Human); 320790 (Mouse)

Gene Symbol

CHD7

UniProt

Q9P2D1 (Human), A2AJK6 (Mouse)

Additional CHD7 Products

Product Documents for CHD7 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CHD7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CHD7 Antibody - BSA Free

Customer Reviews for CHD7 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used CHD7 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • CHD7 Antibody
    Name: Valentina André
    Application: Immunocytochemistry
    Sample Tested: GN11 cell line
    Species: Mouse
    Verified Customer | Posted 09/28/2017
    Fixation: 4% paraformaldehyde in PBS pH 7.4 for 15 minutes at room temperature Permeabilization: PBS containing 0.2% Triton X-100 for 10 minutes Blocking: PBST (PBS+ 0.1% Tween 20) 10% goat serum for 60 minutes Immunostaining: primary Ab 1:100 in PBST in a humidified chamber overnight at 4°C
    CHD7 Antibody - BSA Free NBP1-77393

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

View specific protocols for CHD7 Antibody - BSA Free (NBP1-77393):

CHD7 Antibody:
Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

CHD7 Antibody:
Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

CHD7 Antibody:
Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.

*Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CHD7 Antibody - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I am working with Chd7 and have just visited your website. I would like to have some advices or protocols to use the NBP1-77393 Ab, especially western blot protocol/extraction buffers. My concern is that Chd7 is a high molecular weight protein and it seems very difficult to extract it and to transfer it on a membrane I was just wondering if you had any tips regarding this matter

    A: It was a 5% gel ran 100V for 60 min stressing that it was done in cold conditions with ice packs. It should be ok to do overnight at a lower voltage also in cold conditions. We had the best success on NIH3T3 cells, but also tested on Jurkat. In regards to your inquiry about the Western Blot protocol specific for NBP1-77393, we do have one available on our website. You can find this under the protocol tab. In case you have trouble locating this I will provide it to you in this email. These are just suggestions and some additional optimization may need to be done to achieve the best results based on sample preparation and protein expression. Western Blot Protocol: 1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane. 2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus. 3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate. 4. Rinse the blot. 5. Block the membrane using standard blocking buffer for at least 1 hour. 6. Wash the membrane in wash buffer three times for 10 minutes each. 7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature. 8. Wash the membrane in wash buffer three times for 10 minutes each. 9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature. 10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background). 11. Apply the detection reagent of choice in accordance with the manufacturers instructions. Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies