CHFR Antibody (1H3-A12) - Azide and BSA Free

Novus Biologicals | Catalog # H00055743-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1H3-A12

Format

Azide and BSA Free
View all CHFR Primary Antibodies »

Product Specifications

Immunogen

CHFR (AAH12072.1, 1 a.a. ~ 652 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Reactivity Notes

Human. Other species not tested.

Specificity

CHFR - checkpoint with forkhead and ring finger domains

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CHFR Antibody (1H3-A12) - Azide and BSA Free

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01]

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01]

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.
Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01]

Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01]

Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 2 ug/ml.
Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01]

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01]

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Western Blot analysis of CHFR expression in A-431 ( Cat # L015V1 ).
Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01]

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01]

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( Cat # H00055743-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (Cat # H00055743-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01]

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01]

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12.Lane 1: CHFR transfected lysate (Predicted MW: 72.1 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01]

Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01]

Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue.
Sandwich ELISA: CHFR Antibody (1H3-A12) [H00055743-M01] - Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.

Applications for CHFR Antibody (1H3-A12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.

Reviewed Applications

Read 1 review rated 4 using H00055743-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CHFR

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress

Alternate Names

checkpoint with forkhead and ring finger domains, Checkpoint with forkhead and RING finger domains protein, E3 ubiquitin-protein ligase CHFR, EC 6.3.2.-, FLJ10796, FLJ33629, RING finger protein 196, RNF196RNF116

Entrez Gene IDs

55743 (Human)

Gene Symbol

CHFR

OMIM

605209 (Human)

UniProt

AAH12072 (Human)

Additional CHFR Products

Product Documents for CHFR Antibody (1H3-A12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CHFR Antibody (1H3-A12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CHFR Antibody (1H3-A12) - Azide and BSA Free

Customer Reviews for CHFR Antibody (1H3-A12) - Azide and BSA Free (1)

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  • Name: Motofumi Tanaka
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human colon cancer tissue
    Species: Human
    Verified Customer | Posted 05/12/2010

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