CHIP/STUB1 Antibody (JG38-22)

Novus Biologicals | Catalog # NBP2-75440

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JG38-22 expressed in HEK293
View all CHIP/STUB1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human CHIP/STUB1 aa 10-59 / 303. (SwissProt: Q9UNE7 Human; SwissProt: Q9WUD1 Mouse)

Localization

Nucleus. Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CHIP/STUB1 Antibody (JG38-22)

Western Blot: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Western Blot: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Western Blot: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution.Positive control:Lane 1: 293 Lane 2: MCF-7Lane 3: SK-Br-3 Lane 4: Mouse kidney
Immunocytochemistry/ Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunocytochemistry/ Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded rat epididymis tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
Flow Cytometry: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Flow Cytometry: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Flow Cytometry: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunocytochemistry/ Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunocytochemistry/ Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded human colon cancer tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded human placenta tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440]

Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded mouse kidney tissue using anti-STUB1 antibody. Counter stained with hematoxylin.

Applications for CHIP/STUB1 Antibody (JG38-22)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHIP/STUB1

STUB1/CHIP is an E3 ubiquitin ligase and co-chaperone of heat-shock protein 70 (Hsp70). STUB1/CHIP interacts with Hsp70 to monitor protein folding and direct misfolded proteins for proteasomal degradation. It has been found to also associate with Hsp90 to remove phosphorylated protein tau, a protein that accumulates in the brains of Alzheimers and Down's syndrome patients. STUB1/CHIP has been reported to also have intrinsic chaperone activity and recognize non-native proteins in response to heat stress. In this way, STUB1/CHIP has been shown to be a direct chaperone for p53. Alternate names for STUB1/CHIP include carboxy terminus of Hsp70-interacting protein, STIP1 homology and U-box containing protein 1, CLL-associated antigen KW-8 antigen, serologically defined colon cancer antigen 7, heat shock protein A binding protein 2, NY-CO-7, HSPABP2, and SDCCAG7.

Long Name

C-terminus of Hsc70 Interacting Protein

Alternate Names

HSPABP2, NY-CO-7, SDCCAG7, STUB1, UBOX1

Gene Symbol

STUB1

Additional CHIP/STUB1 Products

Product Documents for CHIP/STUB1 Antibody (JG38-22)

Certificate of Analysis

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Product Specific Notices for CHIP/STUB1 Antibody (JG38-22)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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