Chk2 Antibody (4B7) - Azide and BSA Free

Novus Biologicals | Catalog # H00011200-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B7

Format

Azide and BSA Free
View all Chk2 Primary Antibodies »

Product Specifications

Immunogen

CHEK2 (AAH04207.1, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDAEDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDLLSEENESTALPQVLAQPSTSRKRPREGEAEGAETTKRPAVCAAVL

Reactivity Notes

Human. Other species not tested.

Specificity

CHEK2 - CHK2 checkpoint homolog (S. pombe)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Chk2 Antibody (4B7) - Azide and BSA Free

Western Blot: Chk2 Antibody (4B7) [H00011200-M01]

Western Blot: Chk2 Antibody (4B7) [H00011200-M01]

Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7. Analysis of CHEK2 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (4B7) [H00011200-M01]

Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (4B7) [H00011200-M01]

Immunocytochemistry/Immunofluorescence: Chk2 Antibody (4B7) [H00011200-M01] - Analysis of monoclonal antibody to CHEK2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Chk2 Antibody (4B7) [H00011200-M01]

Immunohistochemistry-Paraffin: Chk2 Antibody (4B7) [H00011200-M01]

Immunohistochemistry-Paraffin: Chk2 Antibody (4B7) [H00011200-M01] - Analysis of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Western Blot: Chk2 Antibody (4B7) [H00011200-M01]

Western Blot: Chk2 Antibody (4B7) [H00011200-M01]

Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7 Analysis of CHEK2 expression in Hela S3 NE.
Sandwich ELISA: Chk2 Antibody (4B7) [H00011200-M01] - Detection limit for recombinant GST tagged CHEK2 is approximately 3ng/ml as a capture antibody.

Applications for Chk2 Antibody (4B7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Chk2

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Long Name

Checkpoint Kinase 2

Alternate Names

CDS1, CHEK2, HuCds1, LFS2, PP1425, Rad53

Entrez Gene IDs

11200 (Human)

Gene Symbol

CHEK2

OMIM

114480 (Human)

UniProt

AAH04207 (Human)

Additional Chk2 Products

Product Documents for Chk2 Antibody (4B7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Chk2 Antibody (4B7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Chk2 Antibody (4B7) - Azide and BSA Free

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Protocols

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FAQs

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