CHMP5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32597

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (94%), Porcine (100%), Rat (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CHMP5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CHMP5. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (88%).

Localization

Cytoplasm, cytosol, Endosome membrane, Peripheral membrane protein, Cytoplasm, cytosol, Endosome membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CHMP5 Antibody - BSA Free

Western Blot: CHMP5 Antibody [NBP1-32597]

Western Blot: CHMP5 Antibody [NBP1-32597]

Western Blot: CHMP5 Antibody [NBP1-32597] - Jurkat whole cell extracts and Jurkat exosome extract (15.4 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP5 antibody [N1C3] diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: CHMP5 Antibody [NBP1-32597]

Immunocytochemistry/ Immunofluorescence: CHMP5 Antibody [NBP1-32597]

Immunocytochemistry/Immunofluorescence: CHMP5 Antibody [NBP1-32597] - HL-60 cells were fixed in ice-cold MeOH for 5 min. Green: CHMP5 protein stained by CHMP5 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: CHMP5 Antibody [NBP1-32597]

Immunohistochemistry-Paraffin: CHMP5 Antibody [NBP1-32597]

Immunohistochemistry-Paraffin: CHMP5 Antibody [NBP1-32597] - Mouse urinary bladder. CHMP5 stained by CHMP5 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: CHMP5 Antibody [NBP1-32597]

Western Blot: CHMP5 Antibody [NBP1-32597]

Western Blot: CHMP5 Antibody [NBP1-32597] - Sample(30ug whole cell lysate)A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 2% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: CHMP5 Antibody [NBP1-32597]

Immunohistochemistry-Paraffin: CHMP5 Antibody [NBP1-32597]

Immunohistochemistry-Paraffin: CHMP5 Antibody [NBP1-32597] - NCIN87 xenograft, using CHMP5 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
CHMP5 Antibody

Western Blot: CHMP5 Antibody [NBP1-32597] -

Western Blot: CHMP5 Antibody [NBP1-32597] -CHMP5 antibody detects CHMP5 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 15 % SDS-PAGE, and blotted with CHMP5 antibody diluted by 1:1000

Applications for CHMP5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CHMP5

CHMP5 is a component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargoes of viral proteins.

Alternate Names

apoptosis-related protein PNAS-2, C9orf83, charged multivesicular body protein 5, chromatin modifying protein 5, Chromatin-modifying protein 5, chromosome 9 open reading frame 83, HSPC177, hVps60, PNAS-2, SNF7 domain containing 2, SNF7 domain-containing protein 2, SNF7DC2, Vacuolar protein sorting-associated protein 60, Vps60CGI-34

Gene Symbol

CHMP5

Additional CHMP5 Products

Product Documents for CHMP5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CHMP5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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