Choline Acetyltransferase/ChAT Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB100-98693

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Format

Azide and BSA Free
View all Choline Acetyltransferase/ChAT Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from mouse Choline Acetyltransferase/ChAT conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

Reactivity Notes

Marmoset

Marker

Cholinergic Neuronal Marker

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Choline Acetyltransferase/ChAT Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

10-50 ug/ml

Immunohistochemistry-Frozen

10-50 ug/ml

Immunohistochemistry-Paraffin

10-50 ug/ml

Western Blot

10-50 ug/ml

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Reconstitution

Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Formulation

Lyophilized from PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Choline Acetyltransferase/ChAT

Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.; Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.; CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine.; Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2); also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.

Alternate Names

CHAT, Choline acetylase

Entrez Gene IDs

12647 (Mouse); 290567 (Rat)

Gene Symbol

CHAT

UniProt

Q03059 (Mouse), P32738 (Rat)

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Choline Acetyltransferase/ChAT Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Choline Acetyltransferase/ChAT Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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