HCG is a glycoprotein hormone secreted in pregnancy by embryonic trophoblast cells during the first 7 weeks of gestation. Production subsequently shifts to the placenta, where it continues until term. HCG is a heterodimer consisting of an alpha chain and a beta chain. The alpha chain is common to several other hormones, including lutenizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), while the beta chain is unique to HCG. Both alpha and beta chains contain a cysteine knot motif, also found in growth factors such as TGF-beta family members.
Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330)
Novus Biologicals | Catalog # NBP2-77412
Recombinant Monoclonal Antibody
![Western Blot: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330) [NBP2-77412]](https://resources.rndsystems.com/images/products/Chorionic-Gonadotropin-alpha-Chain-hCG-alpha-Antibody-RM330-Western-Blot-NBP2-77412-img0002.jpg "Western Blot: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330) [NBP2-77412]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM330 expressed in HEK293
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Product Specifications
Immunogen
Native hCG protein purified from Human Urine
Specificity
This antibody reacts to Chorionic Gonadotropin alpha Chain (hCG alpha). The antibody recognizes both the native complex and the beta subunit of hCG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330)
Western Blot: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330) [NBP2-77412]
Western Blot: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330) [NBP2-77412] - Western Blot of hCG protein purified from human urine using NBP2-77412.![Immunohistochemistry: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330) [NBP2-77412]](https://resources.rndsystems.com/images/products/Chorionic-Gonadotropin-alpha-Chain-hCG-alpha-Antibody-RM330-Immunohistochemistry-NBP2-77412-img0001.jpg "Immunohistochemistry: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330) [NBP2-77412]")
Immunohistochemistry: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330) [NBP2-77412]
Immunohistochemistry: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330) [NBP2-77412] - Immunohistochemical staining of formalin fixed and paraffin embedded human placenta tissue section using NBP2-77412.Applications for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330)
Application
Recommended Usage
ELISA
1:1000 - 1:10000
Immunohistochemistry
1:500 - 1:1000
Western Blot
1:5000 - 1:20000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Chorionic Gonadotropin alpha Chain (HCG alpha)
Alternate Names
alpha HCG, CGA, FSHA, GPHa1, LHA, TSHA
Gene Symbol
CGA
Additional Chorionic Gonadotropin alpha Chain (HCG alpha) Products
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Product Documents for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (RM330)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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