Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-18630

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Chorionic Gonadotropin alpha Chain (HCG alpha) Primary Antibodies »

Product Specifications

Immunogen

Prepared from whole goat serum produced by repeated immunizations with Human Chorionic Gonadotropin alpha Chain (hCG alpha) native protein.

Specificity

This product reacts with Full Human Chorionic Gonadotropin (hCG) and Human Chorionic Gonadotropin Alpha chain (alpha-hCG).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use.

This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Scientific Data Images for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody - BSA Free

Western Blot: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody [NBP3-18630]

Western Blot: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody [NBP3-18630]

Western Blot: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody [NBP3-18630] - Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: hCG alpha (0.05ug) [+]. Lane 3: hCG alpha/U-20S WCL (0.05ug/20ug) [+]. Lane 4: U-20S WCL (20ug) [-]. Lane 5: HeLa WCL (35ug) [+]. Primary Antibody: Anti-alpha-hCG at 1:1000 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG mx8 HRP Conjugated at 1:40,000 for 30 mins at RT. Block: BlockOut Buffer. Exposure: 30 sec. Predicted MW: alpha subunit ~14.9kDa, full hCG ~37kDa, luteinizing hormone (LH) ~29kDa, thyroid stimulating hormone (TSH) ~28kDa, follicle stimulating hormone (FSH) ~30kDa. Observed MW: alpha subunit ~15-20kDa, full hCG ~35kDa.
ELISA: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody [NBP3-18630]

ELISA: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody [NBP3-18630]

ELISA: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody [NBP3-18630] - Each well was coated in duplicate at 10ug/mL of hCG alpha (RED) and full hCG (BLUE) protein. The starting dilution of antibody was 5ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking Buffer, Donkey Anti-Goat IgG MX8 HRP Conjugated Secondary, and TMB ELISA Peroxidase substrate.

Applications for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000
Application Notes
This product has been tested in ELISA and WB. Expect a band at ~14.9kDa for the alpha chain in western blot using appropriate tissues and lysates. ELISA reactivity expected against alpha hCG with an expected titer of ~1:100,000 and full hCG at ~1:50,000 dilution.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Chorionic Gonadotropin alpha Chain (HCG alpha)

HCG is a glycoprotein hormone secreted in pregnancy by embryonic trophoblast cells during the first 7 weeks of gestation. Production subsequently shifts to the placenta, where it continues until term. HCG is a heterodimer consisting of an alpha chain and a beta chain. The alpha chain is common to several other hormones, including lutenizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), while the beta chain is unique to HCG. Both alpha and beta chains contain a cysteine knot motif, also found in growth factors such as TGF-beta family members.

Alternate Names

alpha HCG, CGA, FSHA, GPHa1, LHA, TSHA

Gene Symbol

CGA

UniProt

P01215

Additional Chorionic Gonadotropin alpha Chain (HCG alpha) Products

Product Documents for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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