Chromogranin A Antibody (CL0166) - BSA Free

Novus Biologicals | Catalog # NBP2-52869

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0166

Format

BSA Free
View all Chromogranin A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P10645, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Chromogranin A Antibody (CL0166) - BSA Free

Western Blot: Chromogranin A Antibody (CL0166) [NBP2-52869]

Western Blot: Chromogranin A Antibody (CL0166) [NBP2-52869]

Western Blot: Chromogranin A Antibody (CL0166) [NBP2-52869] - Lane 1: Marker [kDa] Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (CL0166) [NBP2-52869]

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (CL0166) [NBP2-52869]

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody (CL0166) [NBP2-52869] - staining of SH-SY 5Y cells using the Anti-CHGA monoclonal antibody, showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869] - Staining of human duodenum shows strong positivity in a subset of enteroendocrine cells.
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869] - Staining of human colon shows strong reactivity in a subset of enteroendocrine cells.
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869] - Staining of human pancreas shows strong positivity in endocrine islets of Langerhans.

Applications for Chromogranin A Antibody (CL0166) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chromogranin A

Chromogranin A (CgA) is an 86 kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release. Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor a (TNFa) induced gap formation in human umbilical venous endothelial cells. CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody (CL0166) - BSA Free

Certificate of Analysis

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Product Specific Notices for Chromogranin A Antibody (CL0166) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Chromogranin A Antibody (CL0166) - BSA Free

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  • Q: I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?

    A:

    When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

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