Chromogranin A Antibody (CL0166) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-44001
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0166
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P10645, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Chromogranin A Antibody (CL0166) - Azide and BSA Free
![Chromogranin A Antibody (CL0166) - Azide and BSA Free Western Blot: Chromogranin A Antibody [NBP3-44001]](https://resources.rndsystems.com/images/products/nbp3-44001_mouse-chromogranin-a-mab-cl0166-azide-and-bsa-free-282202512173177.jpg "Western Blot: Chromogranin A Antibody [NBP3-44001]")
Western Blot: Chromogranin A Antibody [NBP3-44001]
Lane 1: Marker [kDa]Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Chromogranin A Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
![Chromogranin A Antibody (CL0166) - Azide and BSA Free Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]](https://resources.rndsystems.com/images/products/nbp3-44001_mouse-chromogranin-a-mab-cl0166-azide-and-bsa-free-28220251294024.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]")
![Chromogranin A Antibody (CL0166) - Azide and BSA Free Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]](https://resources.rndsystems.com/images/products/nbp3-44001_mouse-chromogranin-a-mab-cl0166-azide-and-bsa-free-282202512324850.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]")
Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]
Staining of human duodenum shows strong cytoplasmic positivity in enteroendocrine cells.![Chromogranin A Antibody (CL0166) - Azide and BSA Free Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]](https://resources.rndsystems.com/images/products/nbp3-44001_mouse-chromogranin-a-mab-cl0166-azide-and-bsa-free-282202512332919.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]")
Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]
Staining of human liver shows no positivity in hepatocytes as expected.![Chromogranin A Antibody (CL0166) - Azide and BSA Free Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]](https://resources.rndsystems.com/images/products/nbp3-44001_mouse-chromogranin-a-mab-cl0166-azide-and-bsa-free-2822025131546.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]")
Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]
Staining of human pancreas shows moderate to strong cytoplasmic positivity in islets of Langerhans.![Chromogranin A Antibody (CL0166) - Azide and BSA Free Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]](https://resources.rndsystems.com/images/products/nbp3-44001_mouse-chromogranin-a-mab-cl0166-azide-and-bsa-free-28220251353254.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]")
Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP3-44001]
Staining of human parathyroid gland shows strong cytoplasmic positivity in glandular cells.![Chromogranin A Antibody (CL0166) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody [NBP3-44001]](https://resources.rndsystems.com/images/products/nbp3-44001_mouse-chromogranin-a-mab-cl0166-azide-and-bsa-free-282202513415656.jpg "Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody [NBP3-44001]")
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody [NBP3-44001]
Staining of SH-SY 5Y cells using the Anti-Chromogranin A monoclonal antibody).Applications for Chromogranin A Antibody (CL0166) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: Chromogranin A
Alternate Names
Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I
Gene Symbol
CHGA
Additional Chromogranin A Products
Product Documents for Chromogranin A Antibody (CL0166) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Chromogranin A Antibody (CL0166) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Chromogranin A Antibody (CL0166) - Azide and BSA Free
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Q: I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
A:
When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.
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