Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/ICC, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Chromogranin A Primary Antibodies »

Product Specifications

Immunogen

Recombinant full length protein (Human).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27472443).

Localization

Cytoplasmic, Vesicles, Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Chromogranin A Antibody - BSA Free (NB120-15160) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Chromogranin A Antibody: Cited in 41 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Chromogranin A Antibody - BSA Free

Immunohistochemistry-Paraffin: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunohistochemistry-Paraffin: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunohistochemistry-Paraffin: Chromogranin A Antibody [NB120-15160] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse pancreas using Chromogranin A antibody at a dilution of 1:500. The signal was developed using HRP-conjugated anti-rabbit secondary and DAB reagent which followed counterstaining of the section with hematoxylin. The antibody generated a strong staining in Islets of Langerhans. Also, in the periphery of the cells, the pattern of staining was punctate representing the vesicles with Chromogranin A. Cells of lobules and acini showed a weak cytoplasmic and nuclear staining which is potentially the secreted pool of Chromogranin A.
Immunohistochemistry: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunohistochemistry: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunohistochemistry: Chromogranin A Antibody [NB120-15160] - IHC-P analysis fo human pancreas tissue showing specific staining of Chromogranin A in the Islets of Langerhans (dark brown staining).
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160]

Chromogranin-A-Antibody-Immunocytochemistry-Immunofluorescence-NB120-15160-img0007.jpg
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - SH-SY5Y cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Chromogranin A at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Mouse pancreas cryosections stained with insulin (DAKO, Green) and Chromogranin A (NB120-15160; 1:500; White)
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Chromogranin A Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Chromogranin A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] -

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] - ChromograninA positive hormone-negative (CPHN) cells do not replicate during fetal & infant life. Representative pancreatic sections from fetal (A) & infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, & ghrelin) (white), chromograninA (green), Ki67 (red), & DAPI (blue). Yellow arrows indicate CPHN cells. CPHN cells were rarely positive for Ki67 staining in both fetal & infant groups; no detectable difference was found in the frequency of replicative CPHN cells between fetal & infant pancreatic sections. Scale bars: 100 μm for low power & 50 μm for high magnification images. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30687234), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Chromogranin A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] -

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] - ChromograninA positive hormone-negative (CPHN) cells express the beta-cell differentiation transcription factor NKX2.2 in both fetuses & infants. Representative pancreatic sections from fetal (A) & infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, & ghrelin) (white), chromograninA (green), the transcription factor NKX2.2 (red) & DAPI (blue). Yellow arrows indicate CPHN cells. Scale bars: 100 μm for low power & 25 μm for high magnification images. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30687234), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Chromogranin A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] -

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] - ChromograninA positive hormone-negative (CPHN) cells express the endocrine differentiation transcription factor NKX6.1 in both fetal & infant pancreas. Representative pancreatic sections from fetal (A) & infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, & ghrelin) (white), chromograninA (green), the transcription factor NKX6.1 (red) & DAPI (blue). Yellow arrows indicate CPHN cells. Scale bars: 100 μm for low power & 25 μm for high magnification images. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30687234), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Chromogranin A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (PMID 22114116)

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

reported in scientific literature (PMID 32818433)
Application Notes
IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Note: Optimal dilutions/concentrations should be determined by the end user.

Reviewed Applications

Read 2 reviews rated 5 using NB120-15160 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Chromogranin A

Chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Entrez Gene IDs

1113 (Human)

Gene Symbol

CHGA

UniProt

P10645

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Chromogranin A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Chromogranin A Antibody - BSA Free

Customer Reviews for Chromogranin A Antibody - BSA Free (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Chromogranin A Antibody
    Name: Jason Spaeth
    Application: Immunohistochemistry-Frozen
    Sample Tested: Pancreas tissue (cryosection)
    Species: Mouse
    Verified Customer | Posted 11/29/2016
    Mouse pancreas cryosections stained with insulin (DAKO, Green) and Chromogranin A (NB120-15160; 1:500; White)
    Chromogranin A Antibody - BSA Free NB120-15160
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Mouse small intestine and colon
    Species: Mouse
    Verified Customer | Posted 05/07/2012

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Protocols

View specific protocols for Chromogranin A Antibody - BSA Free (NB120-15160):

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Chromogranin A Antibody - BSA Free

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    • Q: I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
      A: When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.
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