CHTF18 Antibody

Novus Biologicals | Catalog # NBP1-03346

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CHTF18 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human chromosome transmission fidelity factor 18 using the numbering given in entry NP_071375.1 (GeneID 63922).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHTF18 Antibody

Western Blot: CHTF18 Antibody [NBP1-03346]

Western Blot: CHTF18 Antibody [NBP1-03346]

Western Blot: CHTF18 Antibody [NBP1-03346] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NBP1-03346 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
Immunohistochemistry-Paraffin: CHTF18 Antibody [NBP1-03346]

Immunohistochemistry-Paraffin: CHTF18 Antibody [NBP1-03346]

Immunohistochemistry-Paraffin: CHTF18 Antibody [NBP1-03346] - Human stomach carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).

Applications for CHTF18 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CHTF18

CTF18 (Chromosome Transmission Fidelity protein 18 homolog) is part of the CTF18-RFC (Replication Factor C) complex composed of CTF18, CTF8, DCC1, and four RFC subunits. This complex has been shown to be important for the establishment of sister chromatid cohesion and functions as a proliferating cell nuclear antigen (PCNA) loader for DNA synthesis. CTF18 is also known as hCTF18, CHTF18, CHL12, and RUVBL.

Alternate Names

C16orf41, C321D2.2, C321D2.3, C321D2.3 (novel protein), C321D2.4 (novel protein), CHL12C321D2.4, chromosome 16 open reading frame 41, chromosome transmission fidelity protein 18 homolog, CTF18, CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae), EC 3.6.1, hCTF18, homolog of yeast CHL12, RUVBL, some homology with holliday junction DNA helicase RUVB like

Entrez Gene IDs

63922 (Human)

Gene Symbol

CHTF18

UniProt

Q8WVB6 (Human)

Additional CHTF18 Products

Product Documents for CHTF18 Antibody

Certificate of Analysis

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Product Specific Notices for CHTF18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CHTF18 Antibody

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Protocols

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