CHTF18 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35785

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CHTF18 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 794-975 of human CHTF18 (NP_071375.1).

Sequence:
GTMLAYSLTYRQERTPDGQYIYRLEPNVEELCRFPELPARKPLTYQTKQLIAREIEVEKMRRAEASARVENSPQVDGSPPGLEGLLGGIGEKGVHRPAPRNHEQRLEHIMRRAAREEQPEKDFFGRVVVRSTAVPSAGDTAPEQDSVERRMGTAVGRSEVWFRFNEGVSNAVRRSLYIRDLL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CHTF18 Antibody - BSA Free

CHTF18 Antibody

Western Blot: CHTF18 Antibody [NBP3-35785] -

Western Blot: CHTF18 Antibody [NBP3-35785] - Western blot analysis of lysates from HeLa cells, using CHTF18 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
CHTF18 Antibody

Immunocytochemistry/ Immunofluorescence: CHTF18 Antibody [NBP3-35785] -

Immunocytochemistry/ Immunofluorescence: CHTF18 Antibody [NBP3-35785] - Immunofluorescence analysis of C6 cells using CHTF18 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for CHTF18 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHTF18

CTF18 (Chromosome Transmission Fidelity protein 18 homolog) is part of the CTF18-RFC (Replication Factor C) complex composed of CTF18, CTF8, DCC1, and four RFC subunits. This complex has been shown to be important for the establishment of sister chromatid cohesion and functions as a proliferating cell nuclear antigen (PCNA) loader for DNA synthesis. CTF18 is also known as hCTF18, CHTF18, CHL12, and RUVBL.

Alternate Names

C16orf41, C321D2.2, C321D2.3, C321D2.3 (novel protein), C321D2.4 (novel protein), CHL12C321D2.4, chromosome 16 open reading frame 41, chromosome transmission fidelity protein 18 homolog, CTF18, CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae), EC 3.6.1, hCTF18, homolog of yeast CHL12, RUVBL, some homology with holliday junction DNA helicase RUVB like

Gene Symbol

CHTF18

Additional CHTF18 Products

Product Documents for CHTF18 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CHTF18 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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