Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4G3
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Product Specifications
Immunogen
Full length human recombinant protein of human CISD1 (NP_060934) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CISD1 Antibody (OTI4G3)
![Western Blot: CISD1 Antibody (OTI4G3) [NBP2-02321]](https://resources.rndsystems.com/images/products/CISD1-Antibody-4G3-Western-Blot-NBP2-02321-img0001.jpg "Western Blot: CISD1 Antibody (OTI4G3) [NBP2-02321]")
Western Blot: CISD1 Antibody (OTI4G3) [NBP2-02321]
Western Blot: CISD1 Antibody (4G3) [NBP2-02321] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1.
Immunocytochemistry/Immunofluorescence: CISD1 Antibody (4G3) [NBP2-02321] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1.
![Immunohistochemistry-Paraffin: CISD1 Antibody (OTI4G3) [NBP2-02321]](https://resources.rndsystems.com/images/products/CISD1-Antibody-4G3-Immunohistochemistry-Paraffin-NBP2-02321-img0004.jpg "Immunohistochemistry-Paraffin: CISD1 Antibody (OTI4G3) [NBP2-02321]")
Immunohistochemistry-Paraffin: CISD1 Antibody (OTI4G3) [NBP2-02321]
Immunohistochemistry-Paraffin: CISD1 Antibody (4G3) [NBP2-02321] - Staining of paraffin-embedded Kidney tissue using anti-CISD1mouse monoclonal antibody.![Immunohistochemistry-Paraffin: CISD1 Antibody (OTI4G3) [NBP2-02321]](https://resources.rndsystems.com/images/products/CISD1-Antibody-4G3-Immunohistochemistry-Paraffin-NBP2-02321-img0002.jpg "Immunohistochemistry-Paraffin: CISD1 Antibody (OTI4G3) [NBP2-02321]")
Immunohistochemistry-Paraffin: CISD1 Antibody (OTI4G3) [NBP2-02321]
Immunohistochemistry-Paraffin: CISD1 Antibody (4G3) [NBP2-02321] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-CISD1 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: CISD1 Antibody (OTI4G3) [NBP2-02321]](https://resources.rndsystems.com/images/products/CISD1-Antibody-4G3-Immunohistochemistry-Paraffin-NBP2-02321-img0003.jpg "Immunohistochemistry-Paraffin: CISD1 Antibody (OTI4G3) [NBP2-02321]")
Immunohistochemistry-Paraffin: CISD1 Antibody (OTI4G3) [NBP2-02321]
Immunohistochemistry-Paraffin: CISD1 Antibody (4G3) [NBP2-02321] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CISD1 mouse monoclonal antibody.Applications for CISD1 Antibody (OTI4G3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.68 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CISD1
Alternate Names
C10orf70, CDGSH iron sulfur domain 1, CDGSH iron sulfur domain-containing protein 1, CDGSH iron-sulfur domain-containing protein 1, chromosome 10 open reading frame 70, MDS029, MGC14684, MitoNEET, ZCD1, zinc finger CDGSH-type domain 1, zinc finger, CDGSH-type domain 1
Entrez Gene IDs
902 (Human)
Gene Symbol
CISD1
Additional CISD1 Products
Product Documents for CISD1 Antibody (OTI4G3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CISD1 Antibody (OTI4G3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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