Claudin-1 Antibody (1C5-D9) - Azide and BSA Free

Novus Biologicals | Catalog # H00009076-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Monkey

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C5-D9

Format

Azide and BSA Free
View all Claudin-1 Primary Antibodies »

Product Specifications

Immunogen

CLDN1 (AAH12471.1, 1 a.a. ~ 211 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV

Specificity

CLDN1 - claudin 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Claudin-1 Antibody (1C5-D9) - Azide and BSA Free

Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in A-431.
Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 5 ug/ml.
Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in PRC/PRF/5.
Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M01), clone 1C5-D9.Lane 1: CLDN1 transfected lysate(23 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Immunoprecipitation: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

Immunoprecipitation: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

Immunoprecipitation: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of CLDN1 transfected lysate using anti-CLDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLDN1 MaxPab rabbit polyclonal antibody.
ELISA: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

ELISA: Claudin-1 Antibody (1C5-D9) [H00009076-M01]

ELISA: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Detection limit for recombinant GST tagged CLDN1 is approximately 0.03ng/ml as a capture antibody.

Applications for Claudin-1 Antibody (1C5-D9) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:31769164).

Reviewed Applications

Read 2 reviews rated 5 using H00009076-M01 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Claudin-1

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Alternate Names

Claudin1, CLD1, CLDN1, SEMP1

Entrez Gene IDs

9076 (Human)

Gene Symbol

CLDN1

OMIM

603718 (Human)

UniProt

AAH12471 (Human)

Additional Claudin-1 Products

Product Documents for Claudin-1 Antibody (1C5-D9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Claudin-1 Antibody (1C5-D9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Claudin-1 Antibody (1C5-D9) - Azide and BSA Free

Customer Reviews for Claudin-1 Antibody (1C5-D9) - Azide and BSA Free (2)

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Customer Images

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  • Claudin-1 Antibody (1C5-D9)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: pig SC cells
    Species: Pig
    Verified Customer | Posted 06/07/2017
    Monoclonal anti-Claudin-1 antibody (1C5-D9) labeling pig SC cells with Alexa488
    Claudin-1 Antibody (1C5-D9) - Azide and BSA Free H00009076-M01
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: SC cells
    Species: Other
    Verified Customer | Posted 01/07/2016
    Pig SC cells stained with Claudin-1 antibody
    Claudin-1 Antibody (1C5-D9) - Azide and BSA Free H00009076-M01

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Protocols

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FAQs for Claudin-1 Antibody (1C5-D9) - Azide and BSA Free

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  • Q: We are looking for two corresponding products, claudin 1 antibody and a peptide which can blocks the CLDN1.Could you plase kindly provide us solution for it? We found at your website one product, namely Claudin 1 Peptide ( NBP1-77036PEP),which can be used for blocking the activity of CLDN1. For the immunogen, 20 amino acids near the carboxy terminus of CLDN1 was mentioned. We found four Claudin 1 antibody ( NBP1-74546,NBP1-35652,NBP1-74517,H00009076-M1),and the immunogen was again the C-terminus of the Claudin 1.Cold you please send me more information about this C-terminus sequence?Is it the same in case of the NBP1-77036PEP and the four mentined antibodies?

    A: The blocking peptide is a good purchase. The epitope for NBP1-35652 has not been mapped and the immunogen sequence is confidential, it is contained within the range between aa 140-211 of O95832 (Uniprot). Unfortunately NBP1-74517 has been discontinued. The amino acid sequence for H00009076-M01 is (AAH12471.1, 1 a.a. ~ 211 a.a). Unfortunately the lab that provides NBP1-74546 will not provide me with an aa range for the epitope as this is proprietary information that they are not willing to pass on.

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