Claudin-14 Antibody

Novus Biologicals | Catalog # NB100-1070

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Claudin-14 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SATHSGYRLNDYV corresponding to C-Terminus according to NP_036262.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Porcine, Bovine. Mouse reactivity reported in scientific literature (PMID: 21454667).

Localization

Cell Membrane

Specificity

Reported variants represent identical protein (NP_036262.1; NP_652763.1; NP_001139551.1; NP_001139550.1 and NP_001139549.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Claudin-14 Antibody

Western Blot: Claudin-14 Antibody [NB100-1070]

Western Blot: Claudin-14 Antibody [NB100-1070]

Western Blot: Claudin-14 Antibody [NB100-1070] - Staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Claudin-14 Antibody

Application
Recommended Usage

ELISA

1:4000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 23 kDa band observed in human liver lysate (calculated MW of 25.7 kDa band according to NP_652763.1). IHC successful usage on mouse heart.
IHC: This antibody has been successfully used in IHC on mouse heart. Use in IHC-P reported in scientific literature (PMID: 21454667).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Claudin-14

CLDN14 is a component of tight junction (TJ) strands. Defects in CLDN14 are the cause of an autosomal recessive form of nonsyndromic sensorineural deafness (DFNB29).

Alternate Names

Claudin14, CLDN14, DFNB29

Entrez Gene IDs

23562 (Human); 56173 (Mouse)

Gene Symbol

CLDN14

UniProt

O95500 (Human)

Additional Claudin-14 Products

Product Documents for Claudin-14 Antibody

Certificate of Analysis

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Product Specific Notices for Claudin-14 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Claudin-14 Antibody

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Protocols

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