Claudin-3 Antibody (CL13130) - Azide and BSA Free

Name: Claudin-3 Antibody (CL13130) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-44366
Price: 589 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-44366

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CL13130

Format

Azide and BSA Free

View all Claudin-3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of O15551, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Claudin-3 Antibody (CL13130) - Azide and BSA Free

Immunohistochemistry-Paraffin: Claudin-3 Antibody [NBP3-44366]

Analysis in human prostate and placenta tissues using NBP3-44366 antibody. Corresponding Claudin-3 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Claudin-3 Antibody [NBP3-44366]

Staining of human colon shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Claudin-3 Antibody [NBP3-44366]

Staining of human colorectal cancer shows strong membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: Claudin-3 Antibody [NBP3-44366]

Staining of human placenta shows no positivity in trophoblastic cells as expected.

Immunohistochemistry-Paraffin: Claudin-3 Antibody [NBP3-44366]

Staining of human ovarian cancer (clear cell carcinoma) shows strong membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: Claudin-3 Antibody [NBP3-44366]

Staining of human ovarian cancer (papillary carcinoma) shows strong membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: Claudin-3 Antibody [NBP3-44366]

Staining of human prostate shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Claudin-3 Antibody [NBP3-44366]

Staining of human ovarian cancer (endometrioid carcinoma) shows strong membranous positivity in tumor cells.

Western Blot: Claudin-3 Antibody [NBP3-44366]

Analysis in human cell line MCF-7 and human cell line SK-MEL-30.

Immunocytochemistry/ Immunofluorescence: Claudin-3 Antibody [NBP3-44366]

Staining of MCF-7 cells using the Anti-Claudin-3 monoclonal antibody).

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Applications for Claudin-3 Antibody (CL13130) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml

Application Notes

For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Claudin-3

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Alternate Names

Claudin3, CLDN3, CPE-R 2, CPETR2, HRVP1

Gene Symbol

CLDN3

Additional Claudin-3 Products

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Product Specific Notices for Claudin-3 Antibody (CL13130) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.