Cleavage stimulation factor 2 Antibody

Novus Biologicals | Catalog # NB100-57530

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Cleavage stimulation factor 2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 375 and 425 of human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa using the numbering given in entry NP_001316.1 (GeneID 1478).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cleavage stimulation factor 2 Antibody

Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57530]

Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57530]

Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57530] - Detection of human and mouse Cleavage stimulation factor 2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSTF64 antibody NB100-57530 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: Cleavage stimulation factor 2 Antibody [NB100-57530]

Immunohistochemistry: Cleavage stimulation factor 2 Antibody [NB100-57530]

Immunohistochemistry: Cleavage stimulation factor 2 Antibody [NB100-57530] - Detection of human CSTF64 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CSTF64 (NB100-57530) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunoprecipitation: Cleavage stimulation factor 2 Antibody [NB100-57530]

Immunoprecipitation: Cleavage stimulation factor 2 Antibody [NB100-57530]

Immunoprecipitation: Cleavage stimulation factor 2 Antibody [NB100-57530] - Detection of human CSTF64 (Cleavage stimulation factor 2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSTF64 antibody NB100-57530 used for IP at 3 ug per reaction. CSTF64 was also immunoprecipitated by rabbit anti-CSTF64 antibody NB100-57531. For blotting immunoprecipitated CSTF64, NB100-57530 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Cleavage stimulation factor 2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:2000-1:10000
Application Notes
This antibody is useful in Western Blot and immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cleavage stimulation factor 2

This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Alternate Names

betaCstF-64 variant 2, CF-1 64 kDa subunit, Cleavage stimulation factor 64 kDa subunit, cleavage stimulation factor subunit 2, cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD, cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, CSTF 64 kDa subunit, CstF-64

Entrez Gene IDs

1478 (Human)

Gene Symbol

CSTF2

UniProt

NP_001316.1 (Human)

Additional Cleavage stimulation factor 2 Products

Product Documents for Cleavage stimulation factor 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cleavage stimulation factor 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cleavage stimulation factor 2 Antibody

There are currently no reviews for this product. Be the first to review Cleavage stimulation factor 2 Antibody and earn rewards!

Have you used Cleavage stimulation factor 2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies