CLIC3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89465

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (91%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CLIC3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MVLLLKGVPFTLTTVDTRRSPDVLKDFAPGSQLPILLYDSDAKTDTLQIEDFLEETLGPPDFPSLAPRYRESNTAGNDVFHKFSAFIKNPVPAQDEALYQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CLIC3 Antibody - BSA Free

Western Blot: CLIC3 Antibody [NBP1-89465] -

Western Blot: CLIC3 Antibody [NBP1-89465] - Analysis in human cell lines MCF-7 and A-549 using anti-CLIC3 antibody. Corresponding CLIC3 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: CLIC3 Antibody [NBP1-89465]

Immunocytochemistry/ Immunofluorescence: CLIC3 Antibody [NBP1-89465]

Immunocytochemistry/Immunofluorescence: CLIC3 Antibody [NBP1-89465] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies. Antibody staining is shown in green.
Simple Western: CLIC3 Antibody [NBP1-89465]

Simple Western: CLIC3 Antibody [NBP1-89465]

Simple Western: CLIC3 Antibody [NBP1-89465] - Simple Western lane view shows a specific band for CLIC3 in 0.2 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: CLIC3 Antibody [NBP1-89465]

Simple Western: CLIC3 Antibody [NBP1-89465]

Simple Western: CLIC3 Antibody [NBP1-89465] - Electropherogram image of the corresponding Simple Western lane view. CLIC3 antibody was used at 1:20 dilution on MCF-7 lysate(s) respectively.

Applications for CLIC3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 lysate(s), separated by Size, antibody dilution of 1:20, apparent MW was 33 kDa.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CLIC3

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 3 is a member of the p64 family and is predominantly localized in the nucleus and stimulates chloride ion channel activity. In addition, this protein may participate in cellular growth control, based on its association with ERK7, a member of the MAP kinase family.

Alternate Names

chloride intracellular channel 3, chloride intracellular channel protein 3

Gene Symbol

CLIC3

Additional CLIC3 Products

Product Documents for CLIC3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CLIC3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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