CLK3 Antibody (1F10) - Azide and BSA Free

Novus Biologicals | Catalog # H00001198-M05

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1F10

Format

Azide and BSA Free
View all CLK3 Primary Antibodies »

Product Specifications

Immunogen

CLK3 (AAH02555, 36 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YPSRREPPPRRSRSRSHDRLPYQRRYRERRDSDTYRCEERSPSFGEDYYGPSRSRHRRRSRERGPYRTRKHAHHCHKRRTRSCSSASSRSQQSSKRSSRSV

Specificity

CLK3 - CDC-like kinase 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CLK3 Antibody (1F10) - Azide and BSA Free

Western Blot: CLK3 Antibody (1F10) [H00001198-M05]

Western Blot: CLK3 Antibody (1F10) [H00001198-M05]

Western Blot: CLK3 Antibody (1F10) [H00001198-M05] - Analysis of CLK3 expression in transfected 293T cell line by CLK3 monoclonal antibody (M05), clone 1F10. Lane 1: CLK3 transfected lysatE (58.6 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CLK3 Antibody (1F10) [H00001198-M05]

Immunocytochemistry/ Immunofluorescence: CLK3 Antibody (1F10) [H00001198-M05]

Immunocytochemistry/Immunofluorescence: CLK3 Antibody (1F10) [H00001198-M05] - Analysis of monoclonal antibody to CLK3 on HeLa cell. Antibody concentration 8 ug/mL.
Immunohistochemistry-Paraffin: CLK3 Antibody (1F10) [H00001198-M05]

Immunohistochemistry-Paraffin: CLK3 Antibody (1F10) [H00001198-M05]

Immunohistochemistry-Paraffin: CLK3 Antibody (1F10) [H00001198-M05] - Analysis of monoclonal antibody to CLK3 on FFPE human placenta. Antibody concentration 0.5 ug/mL.
Western Blot: CLK3 Antibody (1F10) [H00001198-M05]

Western Blot: CLK3 Antibody (1F10) [H00001198-M05]

Western Blot: CLK3 Antibody (1F10) [H00001198-M05] - Analysis of CLK3 over-expressed 293 cell line, cotransfected with CLK3 Validated Chimera RNAi or non-transfected control. Blot probed with H00001198-M05. GAPDH (36.1 kDa) used as loading control.
Western Blot: CLK3 Antibody (1F10) [H00001198-M05]

Western Blot: CLK3 Antibody (1F10) [H00001198-M05]

Western Blot: CLK3 Antibody (1F10) [H00001198-M05] - Analysis of CLK3 expression in HeLa S3 NE (Cat # L013V3).
Western Blot: CLK3 Antibody (1F10) [H00001198-M05]

Western Blot: CLK3 Antibody (1F10) [H00001198-M05]

Western Blot: CLK3 Antibody (1F10) [H00001198-M05] - HeLa cell lysate. Antibody at 1:2000 in 5% BSA-TBST. Detection by Clarity Western ECL (BioRad). Image from verfied customer review.

Applications for CLK3 Antibody (1F10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein and cell lysate for WB. It has been used for IF, RNAi Validation, IHC-P and ELISA.

Reviewed Applications

Read 1 review rated 4 using H00001198-M05 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CLK3

This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Alternatively spliced transcript variants have been described for this gene, but their biological validity has not been determined. Pseudogenes located on chromosomes 1 and 9 have been found for this gene.

Long Name

Dual specificity protein kinase CLK3

Alternate Names

CDC-like kinase 3

Entrez Gene IDs

1198 (Human)

Gene Symbol

CLK3

OMIM

602990 (Human)

UniProt

AAH02555 (Human)

Additional CLK3 Products

Product Documents for CLK3 Antibody (1F10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CLK3 Antibody (1F10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CLK3 Antibody (1F10) - Azide and BSA Free (1)

4 out of 5
1 Customer Rating
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Customer Images

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  • CLK3 Antibody (1F10)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Hela Cell Lysate
    Species: Human
    Verified Customer | Posted 01/03/2019
    Diluted CLK3 antibody (H00001198-M05) at 1:2000 in 5% BSA-TBST and probed the blot overnight at 4 degrees in shaker, followed by three 5 min washes in 1XTBST and probed with anti-Ms secondary at 1:5000, followed by three 5 min washes in 1XTBST and developed using Clarity Western ECL substrate from Bio-Rad. Shown is blot exposed for 7 s.
    CLK3 Antibody (1F10) - Azide and BSA Free H00001198-M05

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Protocols

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FAQs

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