CNPase Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85997

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CNPase Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSKAFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITL

Marker

Oligodendrocyte Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CNPase Antibody - BSA Free

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997] - Staining in human cerebral cortex and pancreas tissues using NBP1-85997 antibody. Corresponding CNP RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997] - Staining of human cerebellum shows strong positivity in neuronal processes in cells in granular layer.
Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997] - Staining of human cerebral cortex shows strong positivity in neuronal processes.
Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85997] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
CNPase Antibody - BSA Free Western Blot: CNPase Antibody - BSA Free [NBP1-85997]

Western Blot: CNPase Antibody - BSA Free [NBP1-85997]

Analysis using Anti-CNP antibody NBP1-85997 (A) shows similar pattern to independent antibody NBP1-85999 (B).
CNPase Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: CNPase Antibody - BSA Free [NBP1-85997]

Immunocytochemistry/ Immunofluorescence: CNPase Antibody - BSA Free [NBP1-85997]

Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Applications for CNPase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CNPase

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Alternate Names

2', 3' cyclic nucleotide 3' phosphohydrolase, 2'-3'-cyclic nucleotide 3' phosphodiesterase, 2'-3'-cyclic-nucleotide 3'-phosphodiesterase, CNP1, CNPase, EC 3.1.4.37

Gene Symbol

CNP

Additional CNPase Products

Product Documents for CNPase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CNPase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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